Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase
文献类型：期刊论文
| 作者 | Tan, Haidong; Li, Kuikui; Chen, Wei; Wang, Wenxia; Li, Shuguang; Yin, Heng |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 201页码:122-130 |
| 关键词 | beta-1 -> Curdlan powder Pre-treatment Glucan oligosaccharide Degradation mode 3-Glucanase |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2018.08.048 |
| 通讯作者 | Yin, Heng(yinheng@dicp.ac.cn) |
| 英文摘要 | Curdlan is a water-insoluble microbial exo-polysaccharide that is hardly degraded. The gene CcGluE encoding an endo-beta-1 -> 3-glucanase consisting of 412 amino acids (44 kDa) from Cellulosimicrobium cellulans E4-5 was cloned and expressed in Escherichia coll. The recombinant CcGluE hydrolysed curdlan powder effectively. CcGluE shows high endo-beta-1 -> 3 glucanase activity and low beta-1,4 and beta-1,6 glucanase activities with broad substrate specificity for glucan, including curdlan, laminarin and beta-1 -> 3/1 -> 6-glucan, and the highest catalytic activity for curdlan. Moreover, the hydrolytic products of curdlan were glucan oligosaccharides with degrees of polymerisation of 2-13, and the main products were glucobiose and glucotriose. Degradation mode analysis indicated that CcGluE is more likely to hydrolyse glucopentaose and revealed that CcGluE was an endo-glucanase. Furthermore, upon combination with a homogenising pre-treatment method with curdlan, the degradation efficiency of CcGluE for curdlan powder was greatly improved 7.1-fold, which laid a good foundation for the utilisation of curdlan. |
| WOS关键词 | EXTRACELLULAR POLYSACCHARIDES ; ESSENTIAL-RESIDUES ; ATCC 31749 ; OLIGOSACCHARIDES ; BETA-1,3-GLUCAN ; ENDO-BETA-1,3-GLUCANASE ; PURIFICATION ; SPECIFICITY ; HYDROLYSIS ; RHIZOBIUM |
| 资助项目 | National Key R&D Programme of China[2017YFD0200900] ; Shaanxi Provincial Key RD Programme[2017TSCXL-NY-02-05] ; National Natural Science Foundation of China[31670803] ; Dalian City Innovative Support Programme for High-Level Talents[2015R010] ; CAS Youth Innovation Promotion Association[2015144] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000445030100014 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association ; National Key R&D Programme of China ; National Key R&D Programme of China ; Shaanxi Provincial Key RD Programme ; Shaanxi Provincial Key RD Programme ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Dalian City Innovative Support Programme for High-Level Talents ; Dalian City Innovative Support Programme for High-Level Talents ; CAS Youth Innovation Promotion Association ; CAS Youth Innovation Promotion Association |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167052]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yin, Heng |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Haidong,Li, Kuikui,Chen, Wei,et al. Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase[J]. CARBOHYDRATE POLYMERS,2018,201:122-130. |
| APA | Tan, Haidong,Li, Kuikui,Chen, Wei,Wang, Wenxia,Li, Shuguang,&Yin, Heng.(2018).Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase.CARBOHYDRATE POLYMERS,201,122-130. |
| MLA | Tan, Haidong,et al."Effective degradation of curdlan powder by a novel endo-beta-1 -> 3-glucanase".CARBOHYDRATE POLYMERS 201(2018):122-130. |
入库方式： OAI收割
来源：大连化学物理研究所
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