The ΔF/Fm′-guided supply of nitrogen in culture medium facilitates sustainable production of TAG in Nannochloropsis oceanica IMET1
文献类型：期刊论文
| 作者 | Cao,Xupeng2; Xue,Song2; Liu,Jiao2,4; Yao,Changhong1; Meng,Yingying2,3; Wu,Peichun2 |
| 刊名 | Biotechnology for Biofuels
 |
| 出版日期 | 2018-06-20 |
| 卷号 | 11期号:1 |
| ISSN号 | 1754-6834 |
| 关键词 | Semi-continuous cultivation ΔF/Fm′ monitoring Nitrogen stress index Microalgae |
| DOI | 10.1186/s13068-018-1168-y |
| 通讯作者 | Xue,Song(xuesong@dicp.ac.cn) |
| 英文摘要 | AbstractBackgroundTriacylglycerol (TAG) from photosynthetic microalgae is a sustainable feedstock for biodiesel production. Physiological stress triggers microalgal TAG accumulation. However excessive physiological stress will impair the photosynthesis system seriously thus decreasing TAG productivity because of the low biomass production. Hence, it is critical to quantitatively and timely monitor the degree of the stress while the microalgal cells growing so that the optimal TAG productivity can be obtained.ResultsThe lack of an on-line monitored indicator has limited our ability to gain knowledge of cellular “health status” information regarding high TAG productivity. Therefore, to monitor the degree of nitrogen stress of the cells, we investigated the correlation between the photosynthetic system II (PS II) quantum yield and the degree of stress based on the high relevancy between photosynthetic reduction and nitrogen stress-induced TAG accumulation in microalgal cells. ΔF/Fm′, which is the chlorophyll fluorescence parameter that reflects the effective capability of PS II, was identified to be a critical factor to indicate the degree of stress of the cells. In addition, the concept of a nitrogen stress index has been defined to quantify the degree of stress. Based on this index and by monitoring ΔF/Fm′ and guiding the supply of nitrogen in culture medium to maintain a stable degree of stress, a stable and efficient semi-continuous process for TAG production has been established.ConclusionThe results indicate that the semi-continuous cultivation process with a controlled degree of stress by monitoring the ΔF/Fm′ indicator will have a significant impact on microalgal TAG production, especially for the outdoor controllable cultivation of microalgae on a large scale. |
| 语种 | 英语 |
| 出版者 | BioMed Central |
| WOS记录号 | BMC:10.1186/S13068-018-1168-Y |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167086]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xue,Song |
| 作者单位 | 1. 2. 3. 4. |
| 推荐引用方式 GB/T 7714 | Cao,Xupeng,Xue,Song,Liu,Jiao,et al. The ΔF/Fm′-guided supply of nitrogen in culture medium facilitates sustainable production of TAG in Nannochloropsis oceanica IMET1[J]. Biotechnology for Biofuels,2018,11(1). |
| APA | Cao,Xupeng,Xue,Song,Liu,Jiao,Yao,Changhong,Meng,Yingying,&Wu,Peichun.(2018).The ΔF/Fm′-guided supply of nitrogen in culture medium facilitates sustainable production of TAG in Nannochloropsis oceanica IMET1.Biotechnology for Biofuels,11(1). |
| MLA | Cao,Xupeng,et al."The ΔF/Fm′-guided supply of nitrogen in culture medium facilitates sustainable production of TAG in Nannochloropsis oceanica IMET1".Biotechnology for Biofuels 11.1(2018). |
入库方式： OAI收割
来源：大连化学物理研究所
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