Rapid transesterification of micro-amount of lipids from microalgae via a micro-mixer reactor
文献类型：期刊论文
| 作者 | Chu,Yadong1; Cao,Xupeng1; Zhao,Yuchao3; Xie,Hua4; Xue,Song1; Liu,Jiao1,2 |
| 刊名 | Biotechnology for Biofuels
 |
| 出版日期 | 2015-12-30 |
| 卷号 | 8期号:1 |
| ISSN号 | 1754-6834 |
| 关键词 | Micro-mixer Fatty acid composition analysis Transesterification Microalgae Micro-scale sample |
| DOI | 10.1186/s13068-015-0416-7 |
| 通讯作者 | Xue,Song(xuesong@dicp.ac.cn) |
| 英文摘要 | AbstractBackgroundFatty acid composition is an important physiological parameter of microalgae, which is taken as the third generation alternative resource of biodiesel. To boost microalgal research and applications, a convenient, rapid, and acid-catalyzed transesterification procedure that satisfies the demand for the analysis of the fatty acid composition of lipids with micro-scale samples in the high-throughput screening of microalgal strains is needed, along with the evaluation of the physiological status of microalgae in response to nutrient stress.ResultsThe reaction conditions of transesterification via a micro-mixer reactor were optimized as follows: 90?°C reaction temperature, 20?min reaction time, 6:1 volume ratio of H2SO4-methanol?to?lipid-in-hexane, and a Y-type micro-mixer with a 20-m-long extended loop that has a 0.3?mm diameter. The minimum amount of sample was decreased to 30?μg lipids. The new approach was successfully applied to the fatty acid composition analysis of soybean oil and microalgal lipids. Definitely, it could be applied to acyl related oils from different sources.ConclusionHere, we have developed a simple and rapid method for the analysis of the fatty acid composition of lipids. The new method requires less than 20?min for transesterification and a minimum of only 30?μg lipid sample. Furthermore, a high-throughput process can be easily realized by numbering up the micro-mixer reactors. The micro-mixer reactor has great potential for applications not only in large-scale biodiesel production but also for the micro-scale analysis of microalgae fatty acid compositions. |
| 语种 | 英语 |
| 出版者 | BioMed Central |
| WOS记录号 | BMC:10.1186/S13068-015-0416-7 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167104]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xue,Song |
| 作者单位 | 1.Chinese Academy of Sciences; Marine Bioengineering Group, Dalian Institute of Chemical Physics 2.University of Chinese Academy of Sciences 3.Chinese Academy of Sciences; Microchemical Engineering and Technology Group, Dalian Institute of Chemical Physics 4.Chinese Academy of Sciences; National Engineering Laboratory for Methanol to Olefins, Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics |
| 推荐引用方式 GB/T 7714 | Chu,Yadong,Cao,Xupeng,Zhao,Yuchao,et al. Rapid transesterification of micro-amount of lipids from microalgae via a micro-mixer reactor[J]. Biotechnology for Biofuels,2015,8(1). |
| APA | Chu,Yadong,Cao,Xupeng,Zhao,Yuchao,Xie,Hua,Xue,Song,&Liu,Jiao.(2015).Rapid transesterification of micro-amount of lipids from microalgae via a micro-mixer reactor.Biotechnology for Biofuels,8(1). |
| MLA | Chu,Yadong,et al."Rapid transesterification of micro-amount of lipids from microalgae via a micro-mixer reactor".Biotechnology for Biofuels 8.1(2015). |
入库方式： OAI收割
来源：大连化学物理研究所
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