Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis
文献类型：期刊论文
| 作者 | Ji, Yibing2; Liu, Tingjiao1; Lin, Bingcheng3; Lu, Yao3; Bai, Ruihan2,3; Li, Linmei3; Liu, Meimei3; Yan, Shiqiang3; Miao, Chunyue2,3; Li, Ruijun2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-05-01 |
| 卷号 | 90期号:9页码:5825-5832 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b00362 |
| 文献子类 | Article |
| 英文摘要 | Despite rapid progresses in single-cell analysis technologies, efforts to control the three-dimensional micro-environment for single cell measurements have been lacking. Here, we report a simple method to incorporate three-dimensional scaffolds, including polyvinylidene fluoride (PVDF) membranes and PVDF membrane replicated analog polydimethylsiloxane, into multiplexed single cell secretomic analysis platforms (including a microwell array and a single cell barcode microchip) to mimic the extracellular physical matrix and mechanical support for single cells. Applying this platform to brain tumor cell line U87 to investigate single cell protein secretion behavior on different substrates, we revealed that single cell protein secretions were regulated differently in three-dimensional (3D) microenvironments. This finding was further verified with intracellular cytokine staining, highlighting the significance of 3D single cell biomimetic platform can be easily adaptable to other three-dimensional cell culture scaffolds or other single cell assays and may become a broadly applicable three-dimensional single cell analysis system to study the effect of microenvironment conditions on cellular functional heterogeneity in vitro. |
| WOS关键词 | DRUG DISCOVERY ; T-CELLS ; CULTURE ; HETEROGENEITY ; CYTOMETRY ; PLATFORM ; MIGRATION ; RESPONSES ; PROTEINS ; MATRICES |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000431464400043 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167154]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ji, Yibing; Lu, Yao |
| 作者单位 | 1.Dalian Med Univ, Coll Stomatol, Dalian 116044, Liaoning, Peoples R China 2.China Pharmaceut Univ, Dept Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Liaoning, Peoples R China 4.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian 116024, Liaoning, Peoples R China 5.Dalian Univ Technol, Sch Pharmaceut Sci & Technol, Dalian 116024, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Yibing,Liu, Tingjiao,Lin, Bingcheng,et al. Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis[J]. ANALYTICAL CHEMISTRY,2018,90(9):5825-5832. |
| APA | Ji, Yibing.,Liu, Tingjiao.,Lin, Bingcheng.,Lu, Yao.,Bai, Ruihan.,...&Luo, Yong.(2018).Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis.ANALYTICAL CHEMISTRY,90(9),5825-5832. |
| MLA | Ji, Yibing,et al."Paper-Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis".ANALYTICAL CHEMISTRY 90.9(2018):5825-5832. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。