Strategy for Comprehensive Identification of Acylcarnitines Based on Liquid Chromatography-High-Resolution Mass Spectrometry
文献类型：期刊论文
| 作者 | Lu, Xin2; Xu, Guowang2; Zhou, Lina2; Xuan, Qiuhui1,2; Yu, Di1,2; Wang, Lichao1,2; Zhao, Xinjie2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-05-01 |
| 卷号 | 90期号:9页码:5712-5718 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b05471 |
| 文献子类 | Article |
| 英文摘要 | Carnitines play important roles in fatty acid oxidation and branched chain amino acid metabolism. The disturbance of acylcarnitines is associated with occurrence and development of many diseases. Comprehensive acylcarnitine identification can greatly benefit their targeted detection, following disease differential diagnosis and possible mechanism study. In this study, we developed a novel strategy to identify as many acylcarnitines as possible based on liquid chromatography-high-resolution mass spectrometry (LC-HRMS). The layer-layer progressive strategy first integrated the initial full scan MS/data-dependent MS/MS monitoring (ddMS(2)) acquisition and the following parallel reaction study monitoring (PRM) to analyze a pooled biological sample. Also 733 possible acylcarnitines were identified containing characteristic high-resolution MS/MS features. Further, accurate mass, retention rules, and HRMS/MS information were used to define subclasses and predict undetected acylcarnitine homologues in each subclass, leading to more acylcarnitines to our newly constructed database. As a result, 758 acylcarnitines were contained in the database, having exact mass, retention time, and MS/MS information, which is the most comprehensive list of acylcarnitines reported to date. Applying this database, 241, 515, and 222 acylcarnitines were rapidly and reliably annotated in human plasma, human urine, and rat liver tissue. This novel strategy enables large-scale identification of acylcarnitines, and a similar method can also be used for identification of other metabolites. |
| WOS关键词 | L-CARNITINE ; INSULIN-RESISTANCE ; BIOLOGICAL SAMPLES ; PLASMA ; METABOLISM ; ATHEROSCLEROSIS ; QUANTIFICATION ; METABOLOMICS ; SEPARATION ; PROTEOMICS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000431464400028 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167204]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xu, Guowang |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Xin,Xu, Guowang,Zhou, Lina,et al. Strategy for Comprehensive Identification of Acylcarnitines Based on Liquid Chromatography-High-Resolution Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2018,90(9):5712-5718. |
| APA | Lu, Xin.,Xu, Guowang.,Zhou, Lina.,Xuan, Qiuhui.,Yu, Di.,...&Zhao, Xinjie.(2018).Strategy for Comprehensive Identification of Acylcarnitines Based on Liquid Chromatography-High-Resolution Mass Spectrometry.ANALYTICAL CHEMISTRY,90(9),5712-5718. |
| MLA | Lu, Xin,et al."Strategy for Comprehensive Identification of Acylcarnitines Based on Liquid Chromatography-High-Resolution Mass Spectrometry".ANALYTICAL CHEMISTRY 90.9(2018):5712-5718. |
入库方式： OAI收割
来源：大连化学物理研究所
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