Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp.
文献类型：期刊论文
| 作者 | Wang, Wenxia2; Yin, Heng2; Yu, Zuochen1,2; Zhu, Benwei3; Tan, Haidong2 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 112页码:937-942 |
| 关键词 | Alginate Monosaccharide Oligoalginate Lyase |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2018.02.046 |
| 文献子类 | Article |
| 英文摘要 | A new oligoalginate lyase encoding gene, designed oal17A, was cloned from marine bacterium Vibrio sp. W13, and then expressed in Escherichia coli. The recombinant Oal17A was purified by NTA-Ni resin with maximal activity at 30 degrees C and pH 7.0. Oal17A exhibited broad substrate specificity, and preferred to degrade alginate than polyM or polyG into monosaccharide acid. The specific activity of Oal17A toward alginate, polyM and polyG was 21.14 U/mg, 12.31 U/mg and 7.43 U/mg, respectively. With features of high-level expression and broad substrate specificity, Oal17A would be a potential tool for alginate monomer production process of alginate utilizing for biofuels and bioethanol production. (C) 2018 Elsevier B.V. All rights reserved. |
| WOS关键词 | ALGINATE LYASE ; AGROBACTERIUM-TUMEFACIENS ; MOLECULAR-IDENTIFICATION ; MAJOR SOURCES ; ACID ; DEPOLYMERIZATION ; METABOLISM ; ATU3025 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000430522400110 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167206]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Yin, Heng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China 3.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wenxia,Yin, Heng,Yu, Zuochen,et al. Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp.[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,112:937-942. |
| APA | Wang, Wenxia,Yin, Heng,Yu, Zuochen,Zhu, Benwei,&Tan, Haidong.(2018).Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp..INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,112,937-942. |
| MLA | Wang, Wenxia,et al."Characterization of a new oligoalginate lyase from marine bacterium Vibrio sp.".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 112(2018):937-942. |
入库方式： OAI收割
来源：大连化学物理研究所
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