Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study
文献类型：期刊论文
| 作者 | Han, Yehua1; Zhou, Zhigui1; Wu, Hongliang1; Nie, Honggang1; Lei, Rong2; Bai, Yu1; Liu, Huwei1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2012-04-27 |
| 卷号 | 1235页码:125-131 |
| 关键词 | Jasmonic Acid Chiral Separation Plant Hormone Liquid Chromatography Quadrupole Time-of-flight Tandem Mass Spectrometry |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2012.02.062 |
| 文献子类 | Article |
| 英文摘要 | Jasmonic acid (JA) is an essential plant hormone involved in plant development and defense system. There are four stereoisorneric forms of JA and they act quite differently in vivo. In this work, a normal phase liquid chromatography-quadrupole time-of-flight mass spectrometry (NPLC-QTOF-MS) method using cellulose tris (4-methylbenzoate) coated silica gel as the chiral stationary phase was first established for the simultaneous discrimination and direct analysis of all the four JA stereoisomers without need of derivatization. A non-endogenous JA stereoisomer was introduced as the internal standard to ensure the reliability of the developed method. Satisfactory results were obtained in terms of sensitivity (limit of detection, 0.5 ng mL(-1) or 2.4 fmol), linearity (R-2 = 0.9996) and repeatability (run-to-run RSD of migration time and peak area, 0.37% and 5.9%, respectively, n=6). Endogenous rise of two natural JA stereoisomers was detected in tobacco leaves and their variations in response to mechanical wounding were monitored. In addition, the configurational stability of JA stereoisomers was investigated using the stereoisomerically pure forms which were not commercially available but easily obtained by our semi-preparative chiral LC method. Experimental evidence indicated that both of the two naturally existing JA stereoisomers were putative signals for wounding response, and the epimerization between them was not a spontaneous process simply promoted by the thermodynamical instability as expected before. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | GENE-EXPRESSION ; METHYL JASMONATE ; PLANT-RESPONSES ; HUMAN PLASMA ; STRESS ; BIOSYNTHESIS ; ARABIDOPSIS ; STEREOISOMERS ; ACCUMULATION ; OXYLIPINS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000302893000014 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167246]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Huwei |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn,Minist Educ,Inst Analyt Chem, Beijing 100871, Peoples R China 2.Chinese Acad Inspect & Quarantine, Inst Plant Quarantine China, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yehua,Zhou, Zhigui,Wu, Hongliang,et al. Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1235:125-131. |
| APA | Han, Yehua.,Zhou, Zhigui.,Wu, Hongliang.,Nie, Honggang.,Lei, Rong.,...&Liu, Huwei.(2012).Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study.JOURNAL OF CHROMATOGRAPHY A,1235,125-131. |
| MLA | Han, Yehua,et al."Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study".JOURNAL OF CHROMATOGRAPHY A 1235(2012):125-131. |
入库方式： OAI收割
来源：大连化学物理研究所
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