中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study

文献类型:期刊论文

作者Han, Yehua1; Zhou, Zhigui1; Wu, Hongliang1; Nie, Honggang1; Lei, Rong2; Bai, Yu1; Liu, Huwei1
刊名JOURNAL OF CHROMATOGRAPHY A
出版日期2012-04-27
卷号1235页码:125-131
关键词Jasmonic Acid Chiral Separation Plant Hormone Liquid Chromatography Quadrupole Time-of-flight Tandem Mass Spectrometry
ISSN号0021-9673
DOI10.1016/j.chroma.2012.02.062
文献子类Article
英文摘要Jasmonic acid (JA) is an essential plant hormone involved in plant development and defense system. There are four stereoisorneric forms of JA and they act quite differently in vivo. In this work, a normal phase liquid chromatography-quadrupole time-of-flight mass spectrometry (NPLC-QTOF-MS) method using cellulose tris (4-methylbenzoate) coated silica gel as the chiral stationary phase was first established for the simultaneous discrimination and direct analysis of all the four JA stereoisomers without need of derivatization. A non-endogenous JA stereoisomer was introduced as the internal standard to ensure the reliability of the developed method. Satisfactory results were obtained in terms of sensitivity (limit of detection, 0.5 ng mL(-1) or 2.4 fmol), linearity (R-2 = 0.9996) and repeatability (run-to-run RSD of migration time and peak area, 0.37% and 5.9%, respectively, n=6). Endogenous rise of two natural JA stereoisomers was detected in tobacco leaves and their variations in response to mechanical wounding were monitored. In addition, the configurational stability of JA stereoisomers was investigated using the stereoisomerically pure forms which were not commercially available but easily obtained by our semi-preparative chiral LC method. Experimental evidence indicated that both of the two naturally existing JA stereoisomers were putative signals for wounding response, and the epimerization between them was not a spontaneous process simply promoted by the thermodynamical instability as expected before. (C) 2012 Elsevier B.V. All rights reserved.
WOS关键词GENE-EXPRESSION ; METHYL JASMONATE ; PLANT-RESPONSES ; HUMAN PLASMA ; STRESS ; BIOSYNTHESIS ; ARABIDOPSIS ; STEREOISOMERS ; ACCUMULATION ; OXYLIPINS
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000302893000014
出版者ELSEVIER SCIENCE BV
源URL[http://cas-ir.dicp.ac.cn/handle/321008/167246]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Liu, Huwei
作者单位1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn,Minist Educ,Inst Analyt Chem, Beijing 100871, Peoples R China
2.Chinese Acad Inspect & Quarantine, Inst Plant Quarantine China, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714
Han, Yehua,Zhou, Zhigui,Wu, Hongliang,et al. Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1235:125-131.
APA Han, Yehua.,Zhou, Zhigui.,Wu, Hongliang.,Nie, Honggang.,Lei, Rong.,...&Liu, Huwei.(2012).Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study.JOURNAL OF CHROMATOGRAPHY A,1235,125-131.
MLA Han, Yehua,et al."Simultaneous determination of jasmonic acid epimers as phytohormones by chiral liquid chromatography-quadrupole time-of-flight mass spectrometry and their epimerization study".JOURNAL OF CHROMATOGRAPHY A 1235(2012):125-131.

入库方式: OAI收割

来源:大连化学物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。