A simple and reusable fluorescent sensor for heme proteins based on a conjugated polymer-doped electrospun nanofibrous membrane
文献类型：期刊论文
| 作者 | Wang, Huaming; Peng, Zhou; Long, Yuanyuan; Chen, Haibo; Yang, Yufei; Li, Na; Liu, Feng |
| 刊名 | TALANTA
 |
| 出版日期 | 2012-05-30 |
| 卷号 | 94页码:216-222 |
| 关键词 | Fluorescent Sensor Electrospun Nanofibrous Membrane Conjugated Polymer Heme Proteins Blood Sample |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2012.03.024 |
| 文献子类 | Article |
| 英文摘要 | We reported a simple and reusable fluorescent sensor for heme proteins based on the electrospun nanofibrous membrane doped with a fluorescent conjugated polymer P. The sensor showed favorable fluorescence sensing performance towards the heme proteins, including hemoglobin (Hb), myoglobin (Mb) and cytochrome c (Cyt c). The surface wettability and sensing performance of the electrospun nanofibrous membrane were investigated in detail using Hb as the model. The nanofibrous sensor showed satisfactory reversibility with less than 10% signal loss after nine quenching-regeneration cycles, and good batch-to-batch reproducibility with a relative standard deviation of 3.4% (n = 3). The linear range of the sensor for Hb determination was 2.0 x 10(-8) to 3.0 x 10(-6) M with a detection limit of 1.2 x 10(-8) M. The quenching process is mainly based on the fluorescence resonance energy transfer mechanism between the fluorescent conjugated polymer P and the heme prosthetic groups, therefore the sensor was selective against most of the common interferents. As an example to evaluate the feasibility of the sensor in practical application, Hb in human blood samples was determined and the results were in good agreement with the data provided by the hospital. To the best of our knowledge, this is the first work using fluorescent electrospun nanofibrous sensor for protein analysis in real biological sample. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | HYDROGEN-PEROXIDE ; HEMOGLOBIN ; PERFORMANCE |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000305167300032 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167270]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Feng |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Huaming,Peng, Zhou,Long, Yuanyuan,et al. A simple and reusable fluorescent sensor for heme proteins based on a conjugated polymer-doped electrospun nanofibrous membrane[J]. TALANTA,2012,94:216-222. |
| APA | Wang, Huaming.,Peng, Zhou.,Long, Yuanyuan.,Chen, Haibo.,Yang, Yufei.,...&Liu, Feng.(2012).A simple and reusable fluorescent sensor for heme proteins based on a conjugated polymer-doped electrospun nanofibrous membrane.TALANTA,94,216-222. |
| MLA | Wang, Huaming,et al."A simple and reusable fluorescent sensor for heme proteins based on a conjugated polymer-doped electrospun nanofibrous membrane".TALANTA 94(2012):216-222. |
入库方式： OAI收割
来源：大连化学物理研究所
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