A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry
文献类型：期刊论文
| 作者 | Huo, Feifeng2; Wang, Xin2; Han, Yehua2; Bai, Yu2; Zhang, Wei2; Yuan, Hancheng1; Liu, Huwei2 |
| 刊名 | TALANTA
 |
| 出版日期 | 2012-09-15 |
| 卷号 | 99页码:420-425 |
| 关键词 | Brassinosteroids Bpba Derivatization Uhplc Triple Quadrupole Mass Spectrometry Arabidopsis Thaliana |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2012.05.073 |
| 文献子类 | Article |
| 英文摘要 | In this work, a new labeling reagent, 2-bromopyridine-5-boronic acid (BPBA), was introduced to derivatize brassinosteroids (BRs). The BPBA not only provided a very simple and rapid labeling procedure, but also remarkably increased the detection sensitivity of BRs. Based on this new labeling reaction, a rapid and sensitive method for BRs' analysis in Arabidopsis thaliana was established by using the ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-QqQ-MS). The extraction and purification procedure of the plant sample was also simplified and improved in this work. Good linearities were obtained for three BRs with the determination coefficients (R-2) about 0.9999. The limits of detection (S/N=3) for three BRs were found to be 2.00-8.00 ng/L while the limits of quantification (S/N=10) were 6.00-23.0 ng/L The RSD % for all three samples are lower than 8.67% (n=5). The recoveries of three BRs spiked in A. thaliana samples were from 76.9% to 86.1%. Using this method, the endogenous 0.055 ng/g fresh weight (FW) 24-epiBR and 0.070 ng/g (RN) 28-epihomoBR were successfully detected from only 2 g A. thaliana plants. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | GAS-CHROMATOGRAPHY ; NATURAL BRASSINOSTEROIDS ; ABSOLUTE-CONFIGURATION ; ENANTIOMERIC EXCESS ; LAMINA INCLINATION ; BETA-LACTAMASE ; SEEDS ; BRASSINOLIDE ; PLANTS ; ACID |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000309785300061 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167272]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Bai, Yu |
| 作者单位 | 1.Waters Corp, Beijing 100026, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huo, Feifeng,Wang, Xin,Han, Yehua,et al. A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry[J]. TALANTA,2012,99:420-425. |
| APA | Huo, Feifeng.,Wang, Xin.,Han, Yehua.,Bai, Yu.,Zhang, Wei.,...&Liu, Huwei.(2012).A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry.TALANTA,99,420-425. |
| MLA | Huo, Feifeng,et al."A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry".TALANTA 99(2012):420-425. |
入库方式： OAI收割
来源：大连化学物理研究所
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