Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling
文献类型:期刊论文
作者 | Chen, Peng R.1,2; Zheng, Siqi2; Zhang, Gong1; Li, Jie2 |
刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION |
出版日期 | 2014-06-01 |
卷号 | 53期号:25页码:6449-6453 |
ISSN号 | 1433-7851 |
关键词 | Anthrax Lethal Factor Bioorthogonal Reactions Genetic Code Expansion Membrane Translocation Protein Labeling |
DOI | 10.1002/anie.201403945 |
文献子类 | Article |
英文摘要 | Coupling the genetic code expansion technique with bioorthogonal reactions enables precise control over the conjugation site as well as the choice of fluorescent probes during protein labeling. However, the advantages of this strategy over bulky and rigid fluorescent proteins (FPs) remain to be fully explored. Here we applied site-specific bioorthogonal labeling on anthrax lethal factor (LF) to visualize its membrane translocation inside live cells. In contrast to the previously reported FP tags that significantly perturbed LF's membrane trafficking, our precisely and quantitatively labeled LF exhibited an endocytic activity comparable to wild-type LF. This allowed time-lapse imaging of LF's natural translocation process from host cell membrane to cytosol, which revealed molecular details of its virulence mechanism. Our strategy is generally applicable for monitoring intracellular protein membrane translocation that is difficult to access using conventional protein labeling methodologies. |
WOS关键词 | AZIDE-ALKYNE CYCLOADDITION ; TOXIN TRANSLOCATION ; FLUORESCENT PROTEIN ; CRYSTAL-STRUCTURE ; PORE ; RECEPTOR ; LONG |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | WILEY-V C H VERLAG GMBH |
WOS记录号 | WOS:000337095900023 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167304] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Chen, Peng R. |
作者单位 | 1.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Synthet & Funct Biomol Ctr,Coll Chem, Beijing 100871, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Peng R.,Zheng, Siqi,Zhang, Gong,et al. Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2014,53(25):6449-6453. |
APA | Chen, Peng R.,Zheng, Siqi,Zhang, Gong,&Li, Jie.(2014).Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,53(25),6449-6453. |
MLA | Chen, Peng R.,et al."Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 53.25(2014):6449-6453. |
入库方式: OAI收割
来源:大连化学物理研究所
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