Study on Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass Spectrometry
文献类型：期刊论文
| 作者 | Liu, Yiqun3; Zhang, Jialing2; Nie, Honggang1; Doug, Chuxia3; Li, Ze2; Zheng, Zhenggao3; Bai, Yu2; Liu, Huwei2; Zhao, Jindong3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014-07-15 |
| 卷号 | 86期号:14页码:7096-7102 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac501596v |
| 文献子类 | Article |
| 英文摘要 | Lipids are important components of cell membranes and thylakoids in cyanobacteria, and they play vital roles in various biological activities. Real-time tracing of the variation of membrane lipids can provide insights of the physiological status of cyanobacterial cells. In this work, easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was utilized to investigate the changes of acidic lipids in unicellular (Synechocystis 6803, Synechococcus 7002) and filamentous (Anabaena 7120) cyanobacteria during different growth phases. A sqdX mutant with a reduced synthesis of sulfoquinovosyl diacylglycerol (SQDG) was constructed to verify the acquired data of EASI-MS. Principal component analysis (PCA) was performed to compare the acquired data, enabling the discrimination of different species of cyanobacteria in day-to-day analysis. The results showed that the three representative cyanobacteria and their growth status can be easily determined on the basis of the lipids components detected by EASI-MS. Very interestingly, significant decreases of the ratios of SQDG/PG and dramatic changes of the unsaturation level of lipids were observed in different culture times in these cyanobacteria, and these two unique characters can be used describe the aging of cyanobacteria. |
| WOS关键词 | DESORPTION ELECTROSPRAY-IONIZATION ; DIATOM THALASSIOSIRA-PSEUDONANA ; SP. PCC 7002 ; FATTY-ACIDS ; SULFOQUINOVOSYL DIACYLGLYCEROL ; PHOTOSYSTEM-II ; THYLAKOID MEMBRANES ; BATCH CULTURE ; REAL-TIME ; PHOSPHATIDYLGLYCEROL |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000339227400052 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167328]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Huwei |
| 作者单位 | 1.Peking Univ, Analyt Instrumentat Ctr, Beijing 100871, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 3.Peking Univ, State Key Lab Prot & Plant Sci, Sch Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yiqun,Zhang, Jialing,Nie, Honggang,et al. Study on Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2014,86(14):7096-7102. |
| APA | Liu, Yiqun.,Zhang, Jialing.,Nie, Honggang.,Doug, Chuxia.,Li, Ze.,...&Zhao, Jindong.(2014).Study on Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass Spectrometry.ANALYTICAL CHEMISTRY,86(14),7096-7102. |
| MLA | Liu, Yiqun,et al."Study on Variation of Lipids during Different Growth Phases of Living Cyanobacteria Using Easy Ambient Sonic-Spray Ionization Mass Spectrometry".ANALYTICAL CHEMISTRY 86.14(2014):7096-7102. |
入库方式： OAI收割
来源：大连化学物理研究所
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