Palladium-triggered deprotection chemistry for protein activation in living cells
文献类型：期刊论文
| 作者 | Zhang, Xiaoyu1; Li, Jie2; Yu, Juntao3; Zhao, Jingyi2; Wang, Jie3; Zheng, Siqi2; Lin, Shixian2; Chen, Long2; Yang, Maiyun2; Jia, Shang2 |
| 刊名 | NATURE CHEMISTRY
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 6期号:4页码:352-361 |
| ISSN号 | 1755-4330 |
| DOI | 10.1038/NCHEM.1887 |
| 文献子类 | Article |
| 英文摘要 | Employing small molecules or chemical reagents to modulate the function of an intracellular protein, particularly in a gain-of-function fashion, remains a challenge. In contrast to inhibitor-based loss-of-function approaches, methods based on a gain of function enable specific signalling pathways to be activated inside a cell. Here we report a chemical rescue strategy that uses a palladium-mediated deprotection reaction to activate a protein within living cells. We identify biocompatible and efficient palladium catalysts that cleave the propargyl carbamate group of a protected lysine analogue to generate a free lysine. The lysine analogue can be genetically and site-specifically incorporated into a protein, which enables control over the reaction site. This deprotection strategy is shown to work with a range of different cell lines and proteins. We further applied this biocompatible protection group/catalyst pair for caging and subsequent release of a crucial lysine residue in a bacterial Type III effector protein within host cells, which reveals details of its virulence mechanism. |
| WOS关键词 | GENETIC-CODE ; MAMMALIAN-CELLS ; PHOSPHOTHREONINE LYASE ; SMALL MOLECULES ; LYSINE ; SITE ; PHOSPHORYLATION ; CATALYSIS ; PATHWAYS ; TYROSINE |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000333396200027 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167344]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Li, Jie |
| 作者单位 | 1.Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China 2.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing Natl Lab Mol Sci,Minist Educ,Coll Chem &, Beijing 100871, Peoples R China 3.Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaoyu,Li, Jie,Yu, Juntao,et al. Palladium-triggered deprotection chemistry for protein activation in living cells[J]. NATURE CHEMISTRY,2014,6(4):352-361. |
| APA | Zhang, Xiaoyu.,Li, Jie.,Yu, Juntao.,Zhao, Jingyi.,Wang, Jie.,...&Chen, Peng R..(2014).Palladium-triggered deprotection chemistry for protein activation in living cells.NATURE CHEMISTRY,6(4),352-361. |
| MLA | Zhang, Xiaoyu,et al."Palladium-triggered deprotection chemistry for protein activation in living cells".NATURE CHEMISTRY 6.4(2014):352-361. |
入库方式： OAI收割
来源：大连化学物理研究所
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