Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection-based CE in bubble cell capillary
文献类型:期刊论文
作者 | Qiao, Liang2; Deng, Yan1,2; Gasilova, Natalia2; Zhou, Ying-Lin1; Zhang, Xin-Xiang1; Girault, Hubert H.2 |
刊名 | ELECTROPHORESIS |
出版日期 | 2014-12-01 |
卷号 | 35期号:23页码:3355-3362 |
ISSN号 | 0173-0835 |
关键词 | Bubble Cell Capillary Ce-electrostatic Spray Ionization Ms Field-enhanced Sample Injection Fluoroquinolones Milk |
DOI | 10.1002/elps.201400294 |
文献子类 | Article |
英文摘要 | Fluoroquinolones are a group of synthetic antibiotics with a broad activity spectrum against mycoplasma, Gram-positive, and Gram-negative bacteria. Due to the extensive use of fluoroquinolones in farming and veterinary science, there is a constant need in the analytical methods able to efficiently monitor their residues in food products of animal origin, regulated by Commission Regulation (European Union) no. 37/2010. Herein, field-enhanced sample injection for sample stacking prior the CZE separation was developed inside a bubble cell capillary for highly sensitive detection of five typical fluoroquinolones in bovine milk. Ethylenediamine was proposed as the main component of BGE for the antibiotics separation. The effect of BGE composition, injection parameters, and water plug length on the field-enhanced sample injection-based CE with UV detection was investigated. Under the optimized conditions, described field-enhanced sample injection-based CE-UV analysis of fluoroquinolones provides LODs varying from 0.4 to 1.3 ng/mL. These LOD values are much lower (from 460 to 1500 times) than those obtained by a conventional CE in a standard capillary without bubble cell. The developed method was finally applied for the analysis of fluoroquinolones in low-fat milk from a Swiss supermarket. Sample recovery values from 93.6 to 106.0% for different fluoroquinolones, and LODs from 0.7 to 2.5 mu g/kg, were achieved. Moreover, the proposed ethylenediamine-based BGE as volatile and compatible with MS system, enabled the coupling of the field-enhanced sample injection-based CE with a recently introduced electrostatic spray ionization MS via an iontophoretic fraction collection interface for qualitative fluoroquinolones identification. |
WOS关键词 | SOLID-PHASE EXTRACTION ; ELECTROPHORESIS-MASS-SPECTROMETRY ; ZONE-ELECTROPHORESIS ; LIQUID CHROMATOGRAPHY ; AMPEROMETRIC DETECTION ; FLUORESCENCE DETECTION ; ANTIBIOTICS ; RESIDUES ; CIPROFLOXACIN ; MS |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | WILEY-BLACKWELL |
WOS记录号 | WOS:000345972900010 |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167350] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Zhang, Xin-Xiang |
作者单位 | 1.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,BNLMS, Beijing 100871, Peoples R China 2.Ecole Polytech Fed Lausanne, Lab Electrochim Phys & Analyt, Lausanne, Switzerland |
推荐引用方式 GB/T 7714 | Qiao, Liang,Deng, Yan,Gasilova, Natalia,et al. Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection-based CE in bubble cell capillary[J]. ELECTROPHORESIS,2014,35(23):3355-3362. |
APA | Qiao, Liang,Deng, Yan,Gasilova, Natalia,Zhou, Ying-Lin,Zhang, Xin-Xiang,&Girault, Hubert H..(2014).Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection-based CE in bubble cell capillary.ELECTROPHORESIS,35(23),3355-3362. |
MLA | Qiao, Liang,et al."Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection-based CE in bubble cell capillary".ELECTROPHORESIS 35.23(2014):3355-3362. |
入库方式: OAI收割
来源:大连化学物理研究所
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