Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples
文献类型：期刊论文
| 作者 | Bai, Yu2; Bai, Yu2,3; Chang, Cuilan2; Du, Fuyou2; Tan, Zhijing1; Liu, Huwei2 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2014-07-01 |
| 卷号 | 35期号:14页码:1984-1988 |
| 关键词 | Capillary Electrophoresis Dynamic Ph Junction Phytohormone Quadrupole Time-of-flight Mass Spectrometry Systemin |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201300434 |
| 文献子类 | Article |
| 英文摘要 | Systemin is an important group of plant peptide hormones participating in the regulation of plant defensive responses. An improved method, based on dynamic pH junction and capillary electrophoresis-quadrupole time-of-flight mass spectrometry, was developed for online enrichment and sensitive determination of trace systemins in plants. After optimization, the online enrichment factors for six target systemins ranged from 90- to 127-fold. The detection limits reached lower than 0.5 nM, which were comparable with the sensitivity of LC-MS method. Satisfactory quantitative results were obtained in terms of linearity (R-2 >= 0.993), dynamic range (3-120 ng/mL), and reproducibility (<= 6.7%). For the analysis of real plant samples, a rapid sample preparation method was developed, using two steps of SPE purification with different retention and separation mechanisms. Finally, this method realized the successful detection of tomato systemin and tobacco hydroxyproline-rich systemin I from plant leaves with shorter analysis time. |
| WOS关键词 | DEFENSE GENE-EXPRESSION ; ONLINE PRECONCENTRATION ; ZONE ELECTROPHORESIS ; WOUND SIGNAL ; PURIFICATION ; NUCLEOTIDES ; STACKING ; TIME |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000339661300007 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167356]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liu, Huwei |
| 作者单位 | 1.Peking Univ, Natl Key Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 3.Natl Inst Clean & Low Carbon Energy NICE, Expt Management Ctr, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Yu,Bai, Yu,Chang, Cuilan,et al. Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples[J]. ELECTROPHORESIS,2014,35(14):1984-1988. |
| APA | Bai, Yu,Bai, Yu,Chang, Cuilan,Du, Fuyou,Tan, Zhijing,&Liu, Huwei.(2014).Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples.ELECTROPHORESIS,35(14),1984-1988. |
| MLA | Bai, Yu,et al."Combination of dynamic pH junction with capillary electrophoresis-mass spectrometry for the determination of systemins in plant samples".ELECTROPHORESIS 35.14(2014):1984-1988. |
入库方式： OAI收割
来源：大连化学物理研究所
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