Modulating Fluorescence Anisotropy of Terminally Labeled Double-Stranded DNA via the Interaction between Dye and Nucleotides for Rational Design of DNA Recognition Based Applications
文献类型：期刊论文
| 作者 | Xu, Xiao2; Xia, Bin1,2,3; Li, Na2; Liu, Feng2; Huang, Hongduan2; Wei, Hejia1,3; Zou, Mingjian2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2015-03-03 |
| 卷号 | 87期号:5页码:2748-2754 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/ac504028n |
| 文献子类 | Article |
| 英文摘要 | Effective signal enhancement for fluorescence anisotropy in a simple manner is most desirable for fluorescence anisotropy method development. This work aimed to provide insights into the fluorescence anisotropy of terminally labeled double-stranded DNA (dsDNA) to facilitate a facile and universal design strategy for DNA recognition based applications. We demonstrated that fluorescence anisotropy of dsDNA could be regulated by the nature of dyes, the molecular volume, and the end structure of dsDNA. Fluorescence anisotropy ascended with the increased number of base pairs up to 18 bp and leveled off thereafter, indicating the molecular volume was not the only factor responsible for fluorescence anisotropy. By choosing dyes with the positively charged center, high fluorescence anisotropy signal was obtained due to the confinement of the segmental motion of dyes through the electrostatic interaction. By properly designing the end structure of dsDNA, fluorescence anisotropy could be further improved by enlarging the effective overall rotational volume, as supported by two-dimensional (2D) H-1-H-1 nuclear Overhauser enhancement spectroscopy (NOESY). With the successful enhancement of the fluorescence anisotropy for terminally labeled dsDNA, simple and universal designs were demonstrated by sensing of major classes of analytes from macromolecules (DNA and protein) to small molecules (cocaine). |
| WOS关键词 | LOCAL CYLINDRICAL SYMMETRY ; SMALL MOLECULES ; POLARIZATION ANISOTROPY ; ROTATIONAL DIFFUSION ; APTAMER ; ASSAY ; MACROMOLECULES ; NANOPARTICLES ; DYNAMICS ; TETRAMETHYLRHODAMINE |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000350611700035 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167372]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xia, Bin |
| 作者单位 | 1.Peking Univ, Beijing NMR Ctr, Beijing 100871, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 3.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Xiao,Xia, Bin,Li, Na,et al. Modulating Fluorescence Anisotropy of Terminally Labeled Double-Stranded DNA via the Interaction between Dye and Nucleotides for Rational Design of DNA Recognition Based Applications[J]. ANALYTICAL CHEMISTRY,2015,87(5):2748-2754. |
| APA | Xu, Xiao.,Xia, Bin.,Li, Na.,Liu, Feng.,Huang, Hongduan.,...&Zou, Mingjian.(2015).Modulating Fluorescence Anisotropy of Terminally Labeled Double-Stranded DNA via the Interaction between Dye and Nucleotides for Rational Design of DNA Recognition Based Applications.ANALYTICAL CHEMISTRY,87(5),2748-2754. |
| MLA | Xu, Xiao,et al."Modulating Fluorescence Anisotropy of Terminally Labeled Double-Stranded DNA via the Interaction between Dye and Nucleotides for Rational Design of DNA Recognition Based Applications".ANALYTICAL CHEMISTRY 87.5(2015):2748-2754. |
入库方式： OAI收割
来源：大连化学物理研究所
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