MEMBRANE AFFINITY-CHROMATOGRAPHY USED FOR THE SEPARATION OF TRYPSIN-INHIBITOR
文献类型：期刊论文
| 作者 | GUO, W; SHANG, ZH; YU, YN; GUAN, YF; ZHOU, LM |
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 1992-03-01 |
| 卷号 | 6期号:2页码:95-98 |
| ISSN号 | 0269-3879 |
| 文献子类 | Article |
| 英文摘要 | Polysulphone (PS) was chemically modified by acrylation-amination and by chloromethylation-amination, respectively. An ultrafiltration membrane of chemically modified polysulphone (CMPS) was prepared by the phase inversion method. Trypsin was then covalently bonded onto the CMPS membrane by diazotization. The activity of immobilized trypsin reaches up to 10200 U/g; 15 mg trypsin was immobilized on 1 g CMPS membrane. Separation of soybean trypsin inhibitor was carried out on the affinity membrane, yielding 6.5 mg pure trypsin inhibitor in one run. The enzyme membrane has good activity and stability. |
| WOS关键词 | PURIFICATION ; IMMOBILIZATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:A1992HL94900010 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167470]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | CHINESE ACAD SCI,DALIAN INST CHEM PHYS,POB 100,DALIAN 116012,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | GUO, W,SHANG, ZH,YU, YN,et al. MEMBRANE AFFINITY-CHROMATOGRAPHY USED FOR THE SEPARATION OF TRYPSIN-INHIBITOR[J]. BIOMEDICAL CHROMATOGRAPHY,1992,6(2):95-98. |
| APA | GUO, W,SHANG, ZH,YU, YN,GUAN, YF,&ZHOU, LM.(1992).MEMBRANE AFFINITY-CHROMATOGRAPHY USED FOR THE SEPARATION OF TRYPSIN-INHIBITOR.BIOMEDICAL CHROMATOGRAPHY,6(2),95-98. |
| MLA | GUO, W,et al."MEMBRANE AFFINITY-CHROMATOGRAPHY USED FOR THE SEPARATION OF TRYPSIN-INHIBITOR".BIOMEDICAL CHROMATOGRAPHY 6.2(1992):95-98. |
入库方式： OAI收割
来源：大连化学物理研究所
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