Hexarelin suppresses cardiac fibroblast proliferation and collagen synthesis in rat
文献类型：期刊论文
| 作者 | Xu, Xiangbin; Pang, Jinjiang; Yin, Hongchao; Li, Meixiu; Hao, Wei; Chen, Chen; Cao, Ji-Min |
| 刊名 | AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 293期号:5页码:H2952-H2958 |
| 关键词 | Growth Hormone Secretagogues Growth Hormone-releasing Peptides Heart |
| ISSN号 | 0363-6135 |
| DOI | 10.1152/ajpheart.00004.2007 |
| 文献子类 | Article |
| 英文摘要 | Hexarelin suppresses cardiac fibroblast proliferation and collagen synthesis in rat. Am J Physiol Heart Circ Physiol 293: H2952-H2958, 2007. First published August 31, 2007; doi:10.1152/ajpheart.00004.2007. - Abnormal growth of cardiac fibroblasts is critically involved in the pathophysiology of cardiac hypertrophy/ remodeling. Hexarelin is a synthetic growth hormone secretagogue (GHS), which possesses a variety of cardiovascular protective activities mediated via the GHS receptor (GHSR), including improving cardiac dysfunction and remodeling. The cellular and molecular mechanisms underlying the effect of GHS on cardiac fibrosis are, however, not clear. In this report, cultured cardiac fibroblasts from 8-day-old rats were stimulated with ANG II or FCS to induce proliferation. The fibroblast proliferation and DNA and collagen synthesis were evaluated utilizing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, H-3-thymidine incorporation, and H-3-proline incorporation. The level of mRNA of transforming growth factor (TGF)-beta was evaluated by RT-PCR, and the active TGF-beta(1) release from cardiac fibroblasts was evaluated by ELISA. The level of cellular cAMP was measured by radioimmunoassay. In addition, the effects of 3,7-dimethyl-l-propargylxanthine ( DMPX; a specific adenosine receptor A(2)R antagonist) and 8-cyclopentyl-1,3- dipropylxanthine ( DPCPX; a specific A(1)R antagonist) were tested. It was found that incubation with 10(-7) mol/l hexarelin for 24 h 1) inhibited the ANG II-induced proliferation and collagen synthesis and the 5% FCS- and TGF-beta-induced increase of DNA synthesis in cardiac fibroblast and 2) reduced ANG II-induced upregulation of TGF-beta mRNA expression and active TGF-beta(1) release from fibroblasts. Hexarelin increased the cellular level of cAMP in cardiac fibroblasts. DMPX (10(-8) mol/l) but not DPCPX abolished the effect of hexarelin on cardiac fibroblast DNA synthesis. It is concluded that hexarelin inhibits DNA and collagen synthesis and proliferation of cardiac fibroblasts through activation of both GHSR and A(2)R and diminishment of ANG II-induced increase in TGF-beta expression and release. |
| WOS关键词 | TRANSFORMING GROWTH FACTOR-BETA(1) ; CONVERTING ENZYME-INHIBITORS ; HORMONE-RELEASING PEPTIDES ; SMOOTH-MUSCLE-CELLS ; ANGIOTENSIN-II ; HEART-FAILURE ; GENE-EXPRESSION ; A(2B) RECEPTORS ; VENTRICULAR DYSFUNCTION ; DNA-SYNTHESIS |
| WOS研究方向 | Cardiovascular System & Cardiology ; Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000251400100040 |
| 出版者 | AMER PHYSIOLOGICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/167660]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Chen, Chen |
| 作者单位 | 1.Jiamusi Univ, Coll Basic Med, Dept Reg Anat, Jiamusi, Peoples R China 2.Prince Henrys Inst Med Res, Clayton, Vic 3168, Australia 3.Chinese Acad Sci, Inst Basic Med Sci, Dept Pathol, Sch Basic Med Sci,Peking Union Med Coll, Beijing 100005, Peoples R China 4.Chinese Acad Sci, Inst Basic Med Sci, Dept Physiol & Pathophysiol, Sch Basic Med Sci,Peking Union Med Coll, Beijing 100005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Xiangbin,Pang, Jinjiang,Yin, Hongchao,et al. Hexarelin suppresses cardiac fibroblast proliferation and collagen synthesis in rat[J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY,2007,293(5):H2952-H2958. |
| APA | Xu, Xiangbin.,Pang, Jinjiang.,Yin, Hongchao.,Li, Meixiu.,Hao, Wei.,...&Cao, Ji-Min.(2007).Hexarelin suppresses cardiac fibroblast proliferation and collagen synthesis in rat.AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY,293(5),H2952-H2958. |
| MLA | Xu, Xiangbin,et al."Hexarelin suppresses cardiac fibroblast proliferation and collagen synthesis in rat".AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 293.5(2007):H2952-H2958. |
入库方式： OAI收割
来源：大连化学物理研究所
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