A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography
文献类型：期刊论文
| 作者 | Cai, Jianfeng1; Cheng, Lingping1; Zhao, Jianchao1; Fu, Qing1; Jin, Yu1; Ke, Yanxiong1; Liang, Xinmiao1,2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2017-11-17 |
| 卷号 | 1524页码:153-159 |
| 关键词 | Hydrophilic Interaction Liquid Chromatography Polyacrylamide Carbohydrates Alcohols |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2017.10.005 |
| 文献子类 | Article |
| 英文摘要 | A hydrophilic interaction liquid chromatography (HILIC) stationary phase was prepared by a two-step synthesis method, immobilizing polyacrylamide on silica sphere particles. The stationary phase (named PA, 5 mu m dia) was evaluated using a mixture of carbohydrates in HILIC mode and the column efficiency reached 121,000 N m(-1). The retention behavior of carbohydrates on PA stationary phase was investigated with three different organic solvents (acetonitrile, ethanol and methanol) employed as the weak eluent. The strongest hydrophilicity of PA stationary phase was observed in both acetonitrile and methanol as the weak eluent, when compared with another two amide stationary phases. Attributing to its high hydrophilicity, three oligosaccharides (xylooligosaccharide, fructooligosaccharide and chitooligosaccharides) presented good retention on PA stationary phase using alcohols/water as mobile phase. Finally, PA stationary phase was successfully applied for the purification of galactooligosaccharides and saponins of Paris polyphylla. It is feasible to use safer and cheaper alcohols to replace acetonitrile as the weak eluent for green analysis and purification of polar compounds on PA stationary phase. (C) 2017 Published by Elsevier B.V. |
| WOS关键词 | LIGHT-SCATTERING DETECTION ; ENE CLICK CHEMISTRY ; VAR. YUNNANENSIS ; POLAR COMPOUNDS ; SAPONINS ; COLUMNS ; UHPLC ; ACID ; HPLC |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000415772100016 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168326]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Jin, Yu; Ke, Yanxiong |
| 作者单位 | 1.East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Jianfeng,Cheng, Lingping,Zhao, Jianchao,et al. A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1524:153-159. |
| APA | Cai, Jianfeng.,Cheng, Lingping.,Zhao, Jianchao.,Fu, Qing.,Jin, Yu.,...&Liang, Xinmiao.(2017).A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1524,153-159. |
| MLA | Cai, Jianfeng,et al."A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1524(2017):153-159. |
入库方式： OAI收割
来源：大连化学物理研究所
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