A robust and effective intact protein fractionation strategy by GO/PEI/Au/PEG nanocomposites for human plasma proteome analysis
文献类型：期刊论文
| 作者 | Deng, Nan1,3; Chen, Yuanbo1,2; Jiang, Bo1; Wu, Qi1; Zhou, Yuan1; Zhang, Xiaodan1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 178页码:49-56 |
| 关键词 | Graphene Oxide Human Plasma Protein Fractionation Low-abundance Proteins |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2017.08.079 |
| 文献子类 | Article |
| 英文摘要 | Identification of human plasma proteins with deep coverage is considered as a great challenge due to its extreme complexity. In this work, an intact proteins fractionation strategy based on multi-interaction between proteins and GO/PEI/Au/PEG nanocomposites (GPAP strategy) was developed for human plasma proteome deep analysis. Compared with untreated method, the number of identified proteins was increased from 858 to 2023, among which the number of low-abundance proteins (< 100 ng/mL) was increased from 2 to 11. The concentration range of the identified proteins was broaden to 9 orders of magnitude. Furthermore, the spectral count of the top three proteins in human plasma (Plasma Albumin, Human IgG, Serotransferrin) were decreased in the range of 37.4-82.6%. An excellent reproducibility of GPAP strategy was verified via stable isotope di methyl label strategy. The functionalized material was demonstrated to be an efficient method to achieve deep coverage identification of human plasma proteome. |
| WOS关键词 | BIOMARKER DISCOVERY ; SELECTIVE ENRICHMENT ; DEPLETION ; SERUM ; FUNCTIONALIZATION ; IDENTIFICATION ; GLYCOPEPTIDES ; LIMITATIONS ; SEPARATION ; LIBRARIES |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000416615500008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168438]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Nan,Chen, Yuanbo,Jiang, Bo,et al. A robust and effective intact protein fractionation strategy by GO/PEI/Au/PEG nanocomposites for human plasma proteome analysis[J]. TALANTA,2018,178:49-56. |
| APA | Deng, Nan.,Chen, Yuanbo.,Jiang, Bo.,Wu, Qi.,Zhou, Yuan.,...&Zhang, Yukui.(2018).A robust and effective intact protein fractionation strategy by GO/PEI/Au/PEG nanocomposites for human plasma proteome analysis.TALANTA,178,49-56. |
| MLA | Deng, Nan,et al."A robust and effective intact protein fractionation strategy by GO/PEI/Au/PEG nanocomposites for human plasma proteome analysis".TALANTA 178(2018):49-56. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。