Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer
文献类型：期刊论文
| 作者 | Zheng Si-Jia1,2; Wang Qing-Qing1,2; Wang Xiao-Lin2; Zhao Xin-Jie2; Xu Guo-Wang2 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 45期号:12页码:1921-1929 |
| 关键词 | Zwitterionic Hydrophilic Interaction Liquid Chromatography/mass Spectrometry Metabolomics Bladder Cancer Polar Metabolites |
| ISSN号 | 0253-3820 |
| DOI | 10.11895/j.issn.0253.3820.171286 |
| 文献子类 | Article |
| 英文摘要 | Bladder cancer (BC) is a fatal malignancy with considerable mortality, and can cause a serious threat to human health. The successful treatment of bladder cancer relies mainly on early detection. Biomarkers are vital to early diagnosis of bladder cancer, and metabonomics play an important role in biomarkers finding. In this study, we used 69 polar metabolites to select the appropriate separation system and develop the zwitterionic hydrophilic chromatography/mass spectrometry (ZIC-HILIC/MS) method. In this method, 50 representative compounds had broad linear ranges between 2-6 orders of magnitude. Moreover the limit of detection of the method was below ng/mL levels. The analysis for six serum samples prepared in parallel showed that this method had good reproducibility, and the RSDs of more than 85% metabolites were less than 30%. Based on this method, it was found that 35 metabolites had significant differences in BC group and healthy control. After screening and validation, the combination of chenodeoxycholic acid, eicosenoic acid, GPC, dodecenoic acid and cystine was a potential biomarker to distinguish BC and normal group. These results indicated that the ZIC. HILIC/MS method could detect diverse metabolites for metabolomic analysis purpose with good reproducibility and stability. |
| WOS关键词 | PLASMA ; URINE |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000419147100013 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168482]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhao Xin-Jie |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng Si-Jia,Wang Qing-Qing,Wang Xiao-Lin,et al. Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(12):1921-1929. |
| APA | Zheng Si-Jia,Wang Qing-Qing,Wang Xiao-Lin,Zhao Xin-Jie,&Xu Guo-Wang.(2017).Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(12),1921-1929. |
| MLA | Zheng Si-Jia,et al."Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry for Serum Metabolomics Analysis of Bladder Cancer".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.12(2017):1921-1929. |
入库方式： OAI收割
来源：大连化学物理研究所
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