Recent advances in methods for the analysis of protein o-glycosylation at proteome level
文献类型：期刊论文
| 作者 | You, Xin1,2; Qin, Hongqiang1,2; Ye, Mingliang1,2 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2018 |
| 卷号 | 41期号:1页码:248-261 |
| ISSN号 | 1615-9306 |
| 关键词 | Acetylgalactosamine Acetylglucosamine Enrichment Methods Glycosylation Proteomics |
| DOI | 10.1002/jssc.201700834 |
| 文献子类 | Review |
| 英文摘要 | O-Glycosylation, which refers to the glycosylation of the hydroxyl group of side chains of Serine/Threonine/Tyrosine residues, is one of the most common post-translational modifications. Compared with N-linked glycosylation, O-glycosylation is less explored because of its complex structure and relatively low abundance. Recently, O-glycosylation has drawn more and more attention for its various functions in many sophisticated biological processes. To obtain a deep understanding of O-glycosylation, many efforts have been devoted to develop effective strategies to analyze the two most abundant types of O-glycosylation, i.e. O-N-acetylgalactosamine and O-N-acetylglucosamine glycosylation. In this review, we summarize the proteomics workflows to analyze these two types of O-glycosylation. For the large-scale analysis of mucin-type glycosylation, the glycan simplification strategies including the "SimpleCell'' technology were introduced. A variety of enrichment methods including lectin affinity chromatography, hydrophilic interaction chromatography, hydrazide chemistry, and chemoenzymatic methodwere introduced for the proteomics analysis of O-N-acetylgalactosamine and O-N-acetylglucosamine glycosylation. |
| WOS关键词 | LINKED N-ACETYLGLUCOSAMINE ; GLCNAC-MODIFIED PROTEINS ; ELECTRON-TRANSFER DISSOCIATION ; PORE COMPLEX GLYCOPROTEINS ; MASS-SPECTROMETRY ; MONOCLONAL-ANTIBODIES ; POSTTRANSLATIONAL MODIFICATIONS ; GLOBAL IDENTIFICATION ; RIBOSOMAL-PROTEINS ; DYNAMIC INTERPLAY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000419514100012 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168498]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ye, Mingliang |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Xin,Qin, Hongqiang,Ye, Mingliang. Recent advances in methods for the analysis of protein o-glycosylation at proteome level[J]. JOURNAL OF SEPARATION SCIENCE,2018,41(1):248-261. |
| APA | You, Xin,Qin, Hongqiang,&Ye, Mingliang.(2018).Recent advances in methods for the analysis of protein o-glycosylation at proteome level.JOURNAL OF SEPARATION SCIENCE,41(1),248-261. |
| MLA | You, Xin,et al."Recent advances in methods for the analysis of protein o-glycosylation at proteome level".JOURNAL OF SEPARATION SCIENCE 41.1(2018):248-261. |
入库方式： OAI收割
来源：大连化学物理研究所
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