Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures
文献类型：期刊论文
| 作者 | Wang, Yayue1,2; Feng, Yanbin1; Cao, Xupeng1; Liu, Yinghui1; Xue, Song1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018-01-23 |
| 卷号 | 8 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-017-19050-x |
| 文献子类 | Article |
| 英文摘要 | D-2-haloacid dehalogenases (D-DEXs) catalyse the hydrolytic dehalogenation of D-2-haloacids, releasing halide ions and producing the corresponding 2-hydroxyacids. A structure-guided elucidation of the catalytic mechanism of this dehalogenation reaction has not been reported yet. Here, we report the catalytic mechanism of a D-DEX, HadD AJ1 from Pseudomonas putida AJ1/23, which was elucidated by X-ray crystallographic analysis and the H-2 O-18 incorporation experiment. HadD AJ1 is an a-helical hydrolase that forms a homotetramer with its monomer including two structurally axisymmetric repeats. The product-bound complex structure was trapped with L-lactic acid in the active site, which is framed by the structurally related helices between two repeats. Site-directed mutagenesis confirmed the importance of the residues lining the binding pocket in stabilizing the enzyme-substrate complex. Asp205 acts as a key catalytic residue and is responsible for activating a water molecule along with Asn131. Then, the hydroxyl group of the water molecule directly attacks the C2 atom of the substrate to release the halogen ion instead of forming an enzyme-substrate ester intermediate as observed in L-2-haloacid dehalogenases. The newly revealed structural and mechanistic information on D-DEX may inspire structure-based mutagenesis to engineer highly efficient haloacid dehalogenases. |
| WOS关键词 | PUTIDA STRAIN AJ1/23 ; DL-2-HALOACID DEHALOGENASE ; L-2-HALOACID DEHALOGENASE ; PURIFICATION ; FOLD ; YL |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000423045400027 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168520]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xue, Song |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yayue,Feng, Yanbin,Cao, Xupeng,et al. Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures[J]. SCIENTIFIC REPORTS,2018,8. |
| APA | Wang, Yayue,Feng, Yanbin,Cao, Xupeng,Liu, Yinghui,&Xue, Song.(2018).Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures.SCIENTIFIC REPORTS,8. |
| MLA | Wang, Yayue,et al."Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures".SCIENTIFIC REPORTS 8(2018). |
入库方式： OAI收割
来源：大连化学物理研究所
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