"Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment
文献类型：期刊论文
| 作者 | Shao, Wenya1,2; Liu, Jianxi1,2; Liang, Yu1; Yang, Kaiguang1; Min, Yi1; Zhang, Xiaodan1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018 |
| 卷号 | 410期号:3页码:1019-1027 |
| 关键词 | Hydrophilic Interaction Chromatography Mixed-mode Retention Mechanism Separation Glycopeptide Enrichment Branched Copolymer |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-017-0626-x |
| 文献子类 | Article |
| 英文摘要 | Three-dimensional branched copolymer, with N,N'-methylene bisacrylamide as the crosslinker and 3-allyloxy-2-hydroxy-1-propane sulfonic acid sodium salt as the monomer, was grafted from silica particles by thiol-ene click reaction. The obtained hydrophilic material with sulfonic acid groups was successfully applied for chromatography separation and glycopeptide enrichment. The separation mechanism was proven as the mixed mode of hydrophilic interaction and cation-exchange by investigating the effect of various chromatographic factors on the retention of polar analytes. By such mixed-mode chromatography, nucleosides, nucleobases, and acidic compounds were successfully separated. The column efficiency was up to 136,000 theoretical plates m(-1) for cytidine, which was much higher than those of previous reports. Furthermore, benefitting from the large amount of hydrophilic groups provided by the branched copolymer, the material was used for the selective enrichment of glycopeptides. Results demonstrated the great potential of such material for chromatography separation and glycoproteome analysis. |
| WOS关键词 | HYDROPHILIC-INTERACTION CHROMATOGRAPHY ; INTERACTION LIQUID-CHROMATOGRAPHY ; MIXED-MODE CHROMATOGRAPHY ; STATIONARY-PHASE ; LINKED GLYCOSYLATION ; MASS-SPECTROMETRY ; PERFORMANCE ; GLYCOPROTEINS ; COMBINATION ; RETENTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000422952600032 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168532]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao, Wenya,Liu, Jianxi,Liang, Yu,et al. "Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(3):1019-1027. |
| APA | Shao, Wenya.,Liu, Jianxi.,Liang, Yu.,Yang, Kaiguang.,Min, Yi.,...&Zhang, Yukui.(2018)."Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(3),1019-1027. |
| MLA | Shao, Wenya,et al.""Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.3(2018):1019-1027. |
入库方式： OAI收割
来源：大连化学物理研究所
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