Panax ginseng genome examination for ginsenoside biosynthesis
文献类型：期刊论文
| 作者 | Xu, Jiang6; Chu, Yang6; Liao, Baosheng6; Xiao, Shuiming6; Yin, Qinggang6; Bai, Rui6; Su, He6,7; Dong, Linlin6; Li, Xiwen6; Qian, Jun6 |
| 刊名 | GIGASCIENCE
 |
| 出版日期 | 2017-10-05 |
| 卷号 | 6期号:11 |
| 关键词 | Panax Ginseng Ginsenosides Genome Mass Spectrometry Imaging |
| ISSN号 | 2047-217X |
| DOI | 10.1093/gigascience/gix093 |
| 文献子类 | Article |
| 英文摘要 | Ginseng, which contains ginsenosides as bioactive compounds, has been regarded as an important traditional medicine for several millennia. However, the genetic background of ginseng remains poorly understood, partly because of the plant's large and complex genome composition. We report the entire genome sequence of Panax ginseng using next-generation sequencing. The 3.5-Gb nucleotide sequence contains more than 60% repeats and encodes 42 006 predicted genes. Twenty-two transcriptome datasets and mass spectrometry images of ginseng roots were adopted to precisely quantify the functional genes. Thirty-one genes were identified to be involved in the mevalonic acid pathway. Eight of these genes were annotated as 3-hydroxy-3-methylglutaryl-CoA reductases, which displayed diverse structures and expression characteristics. A total of 225 UDP-glycosyltransferases (UGTs) were identified, and these UGTs accounted for one of the largest gene families of ginseng. Tandem repeats contributed to the duplication and divergence of UGTs. Molecular modeling of UGTs in the 71st, 74th, and 94th families revealed a regiospecific conserved motif located at the N-terminus. Molecular docking predicted that this motif captures ginsenoside precursors. The ginseng genome represents a valuable resource for understanding and improving the breeding, cultivation, and synthesis biology of this key herb. |
| WOS关键词 | METABOLICALLY ENGINEERED YEASTS ; MULTIPLE SEQUENCE ALIGNMENT ; UDP-GLYCOSYLTRANSFERASES ; FUNCTIONAL-CHARACTERIZATION ; TRITERPENOID SAPONINS ; PHYLOGENETIC ANALYSIS ; MASS-SPECTROMETRY ; READ ALIGNMENT ; TOOL ; IDENTIFICATION |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000419633500001 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168536]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Cheng, Yung-Chi; Chen, Shilin |
| 作者单位 | 1.Peking Univ, Sch Basic Med Sci, Dept Biophys, Hlth Sci Ctr, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China 3.Waters Corp Shanghai Sci & Technol Co Ltd, Shanghai 201206, Peoples R China 4.Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA 5.Chinese Acad Agr Sci, Inst Crop Sci, Key Lab Crop Physiol & Ecol, Minist Agr, Beijing 100081, Peoples R China 6.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China 7.Guangdong Prov Hosp Chinese Med, Guangzhou 510006, Guangdong, Peoples R China 8.China Acad Chinese Med Sci, Natl Data Ctr Tradit Chinese Med, Beijing 100700, Peoples R China 9.China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jiang,Chu, Yang,Liao, Baosheng,et al. Panax ginseng genome examination for ginsenoside biosynthesis[J]. GIGASCIENCE,2017,6(11). |
| APA | Xu, Jiang.,Chu, Yang.,Liao, Baosheng.,Xiao, Shuiming.,Yin, Qinggang.,...&Chen, Shilin.(2017).Panax ginseng genome examination for ginsenoside biosynthesis.GIGASCIENCE,6(11). |
| MLA | Xu, Jiang,et al."Panax ginseng genome examination for ginsenoside biosynthesis".GIGASCIENCE 6.11(2017). |
入库方式： OAI收割
来源：大连化学物理研究所
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