Stimulation of chondrocytes and chondroinduced mesenchymal stem cells by osteoinduced mesenchymal stem cells under a fluid flow stimulus on an integrated microfluidic device
文献类型：期刊论文
| 作者 | Bao, Xuanwen1,2; Li, Zhongyu1; Liu, Hui1; Feng, Ke1; Yin, Fangchao1; Li, Hongjing2; Qin, Jianhua1 |
| 刊名 | MOLECULAR MEDICINE REPORTS
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 17期号:2页码:2277-2288 |
| 关键词 | Microfluidics Mesenchymal Stem Cells Shear Stress Intercellular Interactions Metabolism |
| ISSN号 | 1791-2997 |
| DOI | 10.3892/mmr.2017.8153 |
| 文献子类 | Article |
| 英文摘要 | The aim of the present study was to investigate the stimulation of osteoinduced mesenchymal stem cells (MSCs) into chondrogenically predifferentiated MSCs and chondrocytes in a mechanical environment. A novel two-layer microfluidic chip was used to mimic the interstitial flow in the superficial zones of articular cartilage. The morphology, proliferation rate and the expression of collagen I, collagen II and aggrecan of chondrocytes and chondro-MSCs were investigated. The results revealed that the cells in the bottom layer were influenced by the top layer's osteoinduced MSCs and the bottom layer's shear flow. The expression of collagen I, which may signify the effect of the shear stress on the dedifferentiation change, was weakened by the stimulation of osteoinduced MSCs on the top layer. The expression of collagen II and aggrecan was increased in the fluidic environment by osteoinduced MSCs. These results indicate that osteoinduced MSCs have a significant effect on the phenotype of chondro-MSCs and chondrocytes in the fluidic microenvironment. The present study described a simple and promising way to rapidly evaluate cell responses to other cells in a fluidic environment, which may help to better promote the utilization of MSCs and chondrocytes in tissue engineering. |
| WOS关键词 | IN-VITRO ; CARTILAGE ; SHEAR ; CHONDROGENESIS ; CULTURE ; GROWTH ; DIFFERENTIATION ; HYDROGELS ; REPAIR |
| WOS研究方向 | Oncology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000423224300025 |
| 出版者 | SPANDIDOS PUBL LTD |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168650]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Li, Hongjing; Qin, Jianhua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, 457 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China 2.Dalian Med Univ, Dept Orthopaed, Affiliated Hosp 1, 222 Zhongshan Rd, Dalian 116000, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Xuanwen,Li, Zhongyu,Liu, Hui,et al. Stimulation of chondrocytes and chondroinduced mesenchymal stem cells by osteoinduced mesenchymal stem cells under a fluid flow stimulus on an integrated microfluidic device[J]. MOLECULAR MEDICINE REPORTS,2018,17(2):2277-2288. |
| APA | Bao, Xuanwen.,Li, Zhongyu.,Liu, Hui.,Feng, Ke.,Yin, Fangchao.,...&Qin, Jianhua.(2018).Stimulation of chondrocytes and chondroinduced mesenchymal stem cells by osteoinduced mesenchymal stem cells under a fluid flow stimulus on an integrated microfluidic device.MOLECULAR MEDICINE REPORTS,17(2),2277-2288. |
| MLA | Bao, Xuanwen,et al."Stimulation of chondrocytes and chondroinduced mesenchymal stem cells by osteoinduced mesenchymal stem cells under a fluid flow stimulus on an integrated microfluidic device".MOLECULAR MEDICINE REPORTS 17.2(2018):2277-2288. |
入库方式： OAI收割
来源：大连化学物理研究所
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