Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides
文献类型：期刊论文
| 作者 | Su, Jie1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3 |
| 刊名 | ACS SUSTAINABLE CHEMISTRY & ENGINEERING
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 6期号:2页码:2188-2196 |
| 关键词 | Magnetic Graphene Oxide Mesoporous Silica Adenosine Triphosphate Titanium Ion Phosphopeptide Enrichment |
| ISSN号 | 2168-0485 |
| DOI | 10.1021/acssuschemeng.7b03607 |
| 文献子类 | Article |
| 英文摘要 | Developing an efficient strategy to enrich the low abundance phosphopeptides before mass spectrometry detection is a vital preprocessing step in phosphoproteomics. In this work, we synthesized an adenosine phosphate-Ti4+ functionalized magnetic mesoporous graphene oxide nanocomposite (denoted as MG@ mSiO(2)-ATP-Ti4+) to selectively extract phosphorylated peptides from complex biological samples based on the immobilized metal ion affinity chromatography (IMAC). Mesoporous silica was coated on the substrate material of magnetic graphene oxide and then the ATP containing three phosphate groups was grafted on the inwall of mesoporous channels as chelating ligands to immobilize the Ti4+ cations. With favorable properties, such as large surface area and good hydrophilicity and size-exclusion effect, the MG@mSiO(2)-ATP-Ti4+ exhibited excellent sensitivity and selectivity toward phosphopeptides whether in low concentration of beta-casein digest (20 amol mu L-1, 4 fmol) or the digest mixture of beta-casein and bovine serum albumin (with molar ratio of 1:1000) as well as good reusability. Furthermore, MG@mSiO(2)-ATP-Ti4+ could also be applied in the selective enrichment of phosphorylated peptides from nonfat milk digest and human saliva and serum. |
| WOS关键词 | GRAPHENE/MESOPOROUS SILICA COMPOSITES ; AFFINITY-CHROMATOGRAPHY PLATFORM ; MASS-SPECTROMETRY ANALYSIS ; N-LINKED GLYCAN ; ENDOGENOUS PHOSPHOPEPTIDES ; DESIGNED SYNTHESIS ; PHOSPHOPROTEOME ANALYSIS ; FACILE PREPARATION ; ORGANIC FRAMEWORK ; HYBRID MATERIAL |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000424728300073 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168702]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Chen, Langxing |
| 作者单位 | 1.Nankai Univ, Coll Chem, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Jie,He, Xiwen,Chen, Langxing,et al. Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2018,6(2):2188-2196. |
| APA | Su, Jie,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2018).Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,6(2),2188-2196. |
| MLA | Su, Jie,et al."Adenosine Phosphate Functionalized Magnetic Mesoporous Graphene Oxide Nanocomposite for Highly Selective Enrichment of Phosphopeptides".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 6.2(2018):2188-2196. |
入库方式： OAI收割
来源：大连化学物理研究所
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