SNAP-tag fluorogenic probes for wash free protein labeling
文献类型：期刊论文
| 作者 | Leng, Shuang1,2; Qiao, Qing-Long2,3; Gao, Yue1; Miao, Lu2; Deng, Wu-Guo1; Xu, Zhao-Chao2 |
| 刊名 | CHINESE CHEMICAL LETTERS
 |
| 出版日期 | 2017-10-01 |
| 卷号 | 28期号:10页码:1911-1915 |
| 关键词 | Fluorogenic Probes Wash Free Snap-tag Protein Labeling |
| ISSN号 | 1001-8417 |
| DOI | 10.1016/j.cclet.2017.03.034 |
| 文献子类 | Review |
| 英文摘要 | Protein labeling by using a protein tag and tag-specific fluorescent probes is increasingly becoming a useful technique for the real-time imaging of proteins in living cells. SNAP-tag as one of the most prominent fusion tags has been widely used and already commercially available. Recently, various fluorogenic probes for SNAP-tag based protein labeling were reported. Owing to turn-on fluorescence response, fluorogenic probes for SNAP-tag minimize the fluorescence background caused by unreacted or nonspecifically bound probes and allow for direct imaging in living cells without wash-out steps. Thus, real-time analysis of protein localization, dynamics and interactions has been made possible by SNAP-tag fluorogenic probes. In this review, we describe the design strategies of fluorogenic probes for SNAP-tag and their applications in cellular protein labeling. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved. |
| WOS关键词 | LIVE-CELL ; FLUORESCENT-PROBE ; FUSION PROTEINS ; O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ; DIRECTED EVOLUTION ; LIVING CELLS ; IN-VIVO ; FLUOROPHORE ; TECHNOLOGY ; INDICATORS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000426222000001 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168730]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Miao, Lu; Deng, Wu-Guo; Xu, Zhao-Chao |
| 作者单位 | 1.Dalian Med Univ, Inst Canc Stem Cell, Canc Ctr, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Leng, Shuang,Qiao, Qing-Long,Gao, Yue,et al. SNAP-tag fluorogenic probes for wash free protein labeling[J]. CHINESE CHEMICAL LETTERS,2017,28(10):1911-1915. |
| APA | Leng, Shuang,Qiao, Qing-Long,Gao, Yue,Miao, Lu,Deng, Wu-Guo,&Xu, Zhao-Chao.(2017).SNAP-tag fluorogenic probes for wash free protein labeling.CHINESE CHEMICAL LETTERS,28(10),1911-1915. |
| MLA | Leng, Shuang,et al."SNAP-tag fluorogenic probes for wash free protein labeling".CHINESE CHEMICAL LETTERS 28.10(2017):1911-1915. |
入库方式： OAI收割
来源：大连化学物理研究所
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