Engineering 1-Alkene Biosynthesis and Secretion by Dynamic Regulation in Yeast
文献类型：期刊论文
| 作者 | Zhou, Yongjin J.2,3; Hu, Yating3,4; Zhu, Zhiwei3,4; Siewers, Verena3,4; Nielsen, Jens1,3,4 |
| 刊名 | ACS SYNTHETIC BIOLOGY
 |
| 出版日期 | 2018-02-01 |
| 卷号 | 7期号:2页码:584-590 |
| 关键词 | Yeast Cell Factories Fatty Acids Hydrocarbons Transporter Metabolic Engineering |
| ISSN号 | 2161-5063 |
| DOI | 10.1021/acssynbio.7b00338 |
| 文献子类 | Article |
| 英文摘要 | Microbial production of fatty acid-derived hydrocarbons offers a great opportunity to sustainably supply biofuels and oleochemicals. One challenge is to achieve a high production rate. Besides, low efficiency in secretion will cause high separation costs, and it is therefore desirable to have product secretion. Here, we engineered the budding yeast Saccharomyces cerevisiae to produce and secrete 1-alkenes by manipulation of the fatty acid metabolism, enzyme selection, engineering the electron transfer system and expressing a transporter. Furthermore, we implemented a dynamic regulation strategy to control the expression of membrane enzyme and 1-alkene production and cell growth by relieving the possible toxicity of overexpressed membrane proteins. With these efforts, the engineered yeast cell factory produced 35.3 mg/L 1-alkenes with more than 80% being secreted. This represents a 10-fold improvement compared with earlier reported hydrocarbon production by S. cerevisiae. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; ESCHERICHIA-COLI ; FATTY-ACIDS ; RHODOSPORIDIUM-TORULOIDES ; YARROWIA-LIPOLYTICA ; EXTRACTION ; EXPRESSION ; PATHWAY ; CULTURE ; OLEOCHEMICALS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000426012600029 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168798]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhou, Yongjin J.; Nielsen, Jens |
| 作者单位 | 1.Tech Univ Denmark, Novo Nordisk Fdn, Ctr Biosustainabil, DK-2800 Lyngby, Denmark 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Chalmers Univ Technol, Dept Biol & Biol Engn, Kemivagen 10, SE-41296 Gothenburg, Sweden 4.Chalmers Univ Technol, Ctr Biosustainabil, Novo Nordisk Fdn, SE-41296 Gothenburg, Sweden |
| 推荐引用方式 GB/T 7714 | Zhou, Yongjin J.,Hu, Yating,Zhu, Zhiwei,et al. Engineering 1-Alkene Biosynthesis and Secretion by Dynamic Regulation in Yeast[J]. ACS SYNTHETIC BIOLOGY,2018,7(2):584-590. |
| APA | Zhou, Yongjin J.,Hu, Yating,Zhu, Zhiwei,Siewers, Verena,&Nielsen, Jens.(2018).Engineering 1-Alkene Biosynthesis and Secretion by Dynamic Regulation in Yeast.ACS SYNTHETIC BIOLOGY,7(2),584-590. |
| MLA | Zhou, Yongjin J.,et al."Engineering 1-Alkene Biosynthesis and Secretion by Dynamic Regulation in Yeast".ACS SYNTHETIC BIOLOGY 7.2(2018):584-590. |
入库方式： OAI收割
来源：大连化学物理研究所
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