3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions
文献类型：期刊论文
| 作者 | Wu, Qiong1,2; Jiang, Bo2; Weng, Yejing1,2; Liu, Jianxi2,3; Li, Senwu1,2; Hu, Yechen1,2; Yang, Kaiguang2; Liang, Zhen2; Zhang, Lihua2; Zhang, Yukui2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-02-20 |
| 卷号 | 90期号:4页码:2671-2677 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b04451 |
| 文献子类 | Article |
| 英文摘要 | Boronate affinity materials have been successfully used for the selective recognition of glycoproteins. However, by such materials, the large-scale glycoproteins enrichment from human plasma under physiological conditions is rarely reported. In this work, 3-carboxybenzoboroxole (CBX) functionalized polyethylenimine (PEI) modified magnetic graphene oxide nanocomposites were synthesized. Benefitting from the low pK(a) value of CBX (similar to 6.9) and PEI dendrimer-assisted multivalent binding, the Freundlich constant (K-F) for the adsorption of horseradish peroxidase (HRP) was 3.0-7.3 times higher than that obtained by previous work, displaying the high enrichment capacity. Moreover, PEI could improve the hydrophilicity of nanocomposites and reduce nonglycoprotein adsorption. Therefore, such nanocomposites were successfully applied to the analysis of human plasma glycoproteome under physiological conditions, and the identified glycoproteins number and recognition selectivity was increased when compared to the results obtained by previous boronic acid-functionalized particles (Sil@Poly(APBA-co-MBAAm)) under common alkaline condition (137 vs 78 and 67.8% vs 57.8%, respectively). In addition, thrombin (F2), an important plasma glycoprotein, labile under alkaline conditions, was specifically identified by our method, demonstrating the great promise of such nanocomposites in the deep-coverage glycoproteome analysis. |
| WOS关键词 | DISTILLATION-PRECIPITATION POLYMERIZATION ; SELECTIVE ENRICHMENT ; BORONATE AFFINITY ; MONOLITHIC CAPILLARY ; TRACE GLYCOPROTEINS ; CIS-DIOL ; PH 7.4 ; NANOPARTICLES ; BINDING ; RECOGNITION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000426143100040 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168820]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Fujian Normal Univ, Coll Environm Sci & Engn, Fuzhou 350007, Fujian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Qiong,Jiang, Bo,Weng, Yejing,et al. 3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions[J]. ANALYTICAL CHEMISTRY,2018,90(4):2671-2677. |
| APA | Wu, Qiong.,Jiang, Bo.,Weng, Yejing.,Liu, Jianxi.,Li, Senwu.,...&Zhang, Yukui.(2018).3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions.ANALYTICAL CHEMISTRY,90(4),2671-2677. |
| MLA | Wu, Qiong,et al."3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions".ANALYTICAL CHEMISTRY 90.4(2018):2671-2677. |
入库方式： OAI收割
来源：大连化学物理研究所
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