High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine
文献类型：期刊论文
| 作者 | Wang, Yan1,2; Hua, Lei2; Jiang, Jichun2; Xie, Yuanyuan2; Hou, Keyong2; Li, Qingyun3; Wu, Chenxin1,2; Li, Haiyang2 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2018-05-30 |
| 卷号 | 1008页码:74-81 |
| 关键词 | High-pressure Photon Ionization Mass Spectrometry Dynamic Purge-injection Urine Metabolomics 2 5-dimethylpyrrole |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2018.01.006 |
| 文献子类 | Article |
| 英文摘要 | Small molecule metabolites are widely used as biomarkers in the research field of metabolomics for disease diagnosis and exposure assessment. As a readily available biofluid containing plenty of volatile organic metabolites (VOMs), urine is ideal for non-invasive metabolomic analysis; however, there is still lack of rapid analysis method for VOMs in urine. Here we report a kind of rapid method for urine analysis by employing high-pressure photon ionization time-of-flight mass spectrometry (HPPI-TOFMS) combined with dynamic purge-injection. Various types of metabolites, such as ketones, alcohols, acids, sulfides, pyrroles and amines were detected directly by simple acidification or alkalization of urines. It is noteworthy that nitrogen-containing compounds, especially polar amines, could be ultrasensitively measured without any derivatization. The analytical capability of the direct HPPI-MS technique was demonstrated by analyzing five valuable metabolites, i.e., toluene, 2,5-dimethylpyrrole, trimethlyamine, styrene, and p-xylene, which exhibited relatively low limits of detection, wide linear range and satisfactory repeatability. Being highly sensitive and humidity-friendly, the whole analytical procedure is easily operated in less than 6 min. Interestingly, a new biomarker 2,5-dimethylpyrrole was exclusively found in the smoker's urine sample besides toluene. The work presents a novel tool for rapid nontarget disease biomarkers screening or target monitoring of specific compounds through the investigation of volatile metabolites in urine. (C) 2018 Elsevier B.V. All rights reserved. |
| WOS关键词 | SOLID-PHASE MICROEXTRACTION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TRIMETHYLAMINE-N-OXIDE ; GAS-CHROMATOGRAPHY ; ORGANIC-COMPOUNDS ; CANCER BIOMARKERS ; SOIL SAMPLES ; HS-SPME ; HEADSPACE ; METABOLOMICS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000424539800009 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168836]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Li, Haiyang |
| 作者单位 | 1.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Jilin Univ, Dept Instrumentat & Elect Engn, Changchun 130021, Jilin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yan,Hua, Lei,Jiang, Jichun,et al. High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine[J]. ANALYTICA CHIMICA ACTA,2018,1008:74-81. |
| APA | Wang, Yan.,Hua, Lei.,Jiang, Jichun.,Xie, Yuanyuan.,Hou, Keyong.,...&Li, Haiyang.(2018).High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine.ANALYTICA CHIMICA ACTA,1008,74-81. |
| MLA | Wang, Yan,et al."High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine".ANALYTICA CHIMICA ACTA 1008(2018):74-81. |
入库方式： OAI收割
来源：大连化学物理研究所
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