Profiling of Sialylated Oligosaccharides in Mammalian Milk Using Online Solid Phase Extraction-Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Mass Spectrometry
文献类型：期刊论文
| 作者 | Yan, Jingyu2; Ding, Junjie2,3; Jin, Gaowa2; Yu, Dongping2,3; Yu, Long2; Lone, Zhen1; Guo, Zhimou2; Chai, Wengang4; Liang, Xinmiao2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-03-06 |
| 卷号 | 90期号:5页码:3174-3182 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b04468 |
| 文献子类 | Article |
| 英文摘要 | Sialylated oligosaccharides are important components in mammalian milk. They play a key role in the health and growth of infants by helping to shape up infant's gastrointestinal microbiota and defense against infection by various pathogenic agents. A detailed knowledge of the structures, compositions, and quantities of the sialylated milk oligosaccharides (SMOs) is a prerequisite for understanding their biological roles. However, because of the presence of very large amounts of lactose and neutral oligosaccharides, accurate analysis of SMOs is difficult. A pretreatment step is required to remove lactose and neutral oligosaccharides but conventional off-line pretreatment methods are time-consuming and of poor reproducibility. In this presentation, we linked solid-phase extraction (SPE) with hydrophilic interaction chromatography (HILIC) followed by mass spectrometry (MS) identification for the analysis of SMOs. A SPE column with electrostatic repulsion function was used for removal of lactose and neutral oligosaccharides, a HILIC analytical column for separation of the SMOs, and negative-ion electrospray ionization tandem MS was used for their identification and sequencing. The success of the established online SPE-HILIC-MS method was demonstrated by profiling of SMOs in human to investigate detailed SMO changes during lactation period and in animals to compare the difference in SMO contents among the different species. |
| WOS关键词 | CAPILLARY-ELECTROPHORESIS ; LIQUID-CHROMATOGRAPHY ; ESCHERICHIA-COLI ; PORCINE MILK ; INHIBITION ; BINDING ; SIALYLOLIGOSACCHARIDES ; MICROBIOTA ; LACTATION ; ANIMALS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000427095700030 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168864]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Guo, Zhimou; Chai, Wengang; Liang, Xinmiao |
| 作者单位 | 1.Thermofisher Sci Corp, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Imperial Coll London, Dept Med, Glycosci Lab, Hammersmith Campus,Du Cane Rd, London W12 0NN, England |
| 推荐引用方式 GB/T 7714 | Yan, Jingyu,Ding, Junjie,Jin, Gaowa,et al. Profiling of Sialylated Oligosaccharides in Mammalian Milk Using Online Solid Phase Extraction-Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2018,90(5):3174-3182. |
| APA | Yan, Jingyu.,Ding, Junjie.,Jin, Gaowa.,Yu, Dongping.,Yu, Long.,...&Liang, Xinmiao.(2018).Profiling of Sialylated Oligosaccharides in Mammalian Milk Using Online Solid Phase Extraction-Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Mass Spectrometry.ANALYTICAL CHEMISTRY,90(5),3174-3182. |
| MLA | Yan, Jingyu,et al."Profiling of Sialylated Oligosaccharides in Mammalian Milk Using Online Solid Phase Extraction-Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Mass Spectrometry".ANALYTICAL CHEMISTRY 90.5(2018):3174-3182. |
入库方式： OAI收割
来源：大连化学物理研究所
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