Human brain organoid-on-a-chip to model prenatal nicotine exposure
文献类型：期刊论文
| 作者 | Wang, Yaqing1,2,4; Wang, Li2,4; Zhu, Yujuan1,2,4; Qin, Jianhua1,2,3 |
| 刊名 | LAB ON A CHIP
 |
| 出版日期 | 2018-03-21 |
| 卷号 | 18期号:6页码:851-860 |
| ISSN号 | 1473-0197 |
| DOI | 10.1039/c7lc01084b |
| 文献子类 | Article |
| 英文摘要 | Nicotine has been recognized to trigger various neuronal disabilities in the fetal brain and long-lasting behavioral deficits in offspring. However, further understanding of fetal brain development under nicotine exposure is challenging due to the limitations of existing animal models. Here, we create a new brain organoid-on-a-chip system derived from human induced pluripotent stem cells (hiPSCs) that allows us to model neurodevelopmental disorders under prenatal nicotine exposure (PNE) at early stages. The brain organoid-on-a-chip system facilitates 3D culture, in situ neural differentiation, and self-organization of brain organoids under continuous perfused cultures in a controlled manner. The generated brain organoids displayed well-defined neural differentiation, regionalization, and cortical organization, which recapitulates the key features of the early stages of human brain development. The brain organoids exposed to nicotine exhibited premature neuronal differentiation with enhanced expression of the neuron marker TUJ1. Brain regionalization and cortical development were disrupted in the nicotine-treated organoids identified by the expressions of forebrain (PAX6 and FOXG1), hindbrain (PAX2 and KROX20) and cortical neural layer (preplate TBR1 and deep-layer CTIP2) markers. Moreover, the neurite outgrowth showed abnormal neuronal differentiation and migration in nicotine-treated brain organoids. These results suggest that nicotine exposure elicits impaired neurogenesis in early fetal brain development during gestation. The established brain organoid-on-a-chip system provides a promising platform to model neurodevelopmental disorders under environmental exposure, which can be extended for applications in brain disease studies and drug testing. |
| WOS关键词 | PLURIPOTENT STEM-CELLS ; CEREBRAL ORGANOIDS ; INTERSTITIAL FLOW ; NEOCORTEX ; NEURONS ; TISSUES ; HYPERACTIVITY ; EXPRESSION ; HINDBRAIN ; EVOLUTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000428090800001 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168882]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Qin, Jianhua |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yaqing,Wang, Li,Zhu, Yujuan,et al. Human brain organoid-on-a-chip to model prenatal nicotine exposure[J]. LAB ON A CHIP,2018,18(6):851-860. |
| APA | Wang, Yaqing,Wang, Li,Zhu, Yujuan,&Qin, Jianhua.(2018).Human brain organoid-on-a-chip to model prenatal nicotine exposure.LAB ON A CHIP,18(6),851-860. |
| MLA | Wang, Yaqing,et al."Human brain organoid-on-a-chip to model prenatal nicotine exposure".LAB ON A CHIP 18.6(2018):851-860. |
入库方式： OAI收割
来源：大连化学物理研究所
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