Tuning of acyl-ACP thioesterase activity directed for tailored fatty acid synthesis
文献类型：期刊论文
| 作者 | Feng, Yanbin2; Zhang, Yunxiu1,2; Wang, Yayue1,2; Liu, Jiao1,2; Liu, Yinghui2; Cao, Xupeng2; Xue, Song2 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 102期号:7页码:3173-3182 |
| ISSN号 | 0175-7598 |
| 关键词 | Acyl-acp Thioesterase Medium-chain Fatty Acids Fatty Acid Tailoring Substrate Selectivity Competitive Relationship |
| DOI | 10.1007/s00253-018-8770-6 |
| 文献子类 | Article |
| 英文摘要 | Medium-chain fatty acids have attracted significant attention as sources of biofuels in recent years. Acyl-ACP thioesterase, which is considered as the key enzyme to determine the carbon chain length, catalyzes the termination of de novo fatty acid synthesis. Although recombinant medium-chain acyl-ACP thioesterase (TE) affects the fatty acid profile in heterologous cells, tailoring of the fatty acid composition merely by engineering a specific TE is still intractable. In this study, the activity of a C8-C10-specific thioesterase FatB2 from Cuphea hookeriana on C10-ACP was quantified twice as high as that on C8-ACP based on a synthetic C8-C16 acyl-ACP pool in vitro. Whereas in vivo, it was demonstrated that ChFatB2 preferred to accumulate C8 fatty acids with 84.9% composition in the ChFatB2-engineered E. coli strain. To achieve C10 fatty acid production, ChFatB2 was rationally tuned based on structural investigation and enzymatic analysis. An I198E mutant was identified to redistribute the C8-ACP flow, resulting in C10 fatty acid being produced as the principal component at 57.6% of total fatty acids in vivo. It was demonstrated that the activity of TE relative to beta-ketoacyl-ACP synthases (KAS) directly determined the fatty acid composition. Our results provide a prospective strategy in tailoring fatty acid synthesis by tuning of TE activities based on TE-ACP interaction. |
| WOS关键词 | CARRIER PROTEIN THIOESTERASE ; INCREASED LIPID PRODUCTION ; ESCHERICHIA-COLI ; SUBSTRATE-SPECIFICITY ; MICROBIAL-PRODUCTION ; BIOSYNTHESIS ; EXPRESSION ; DESATURASE ; PLANTS ; MODEL |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000427579600020 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168938]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Xue, Song |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Yanbin,Zhang, Yunxiu,Wang, Yayue,et al. Tuning of acyl-ACP thioesterase activity directed for tailored fatty acid synthesis[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2018,102(7):3173-3182. |
| APA | Feng, Yanbin.,Zhang, Yunxiu.,Wang, Yayue.,Liu, Jiao.,Liu, Yinghui.,...&Xue, Song.(2018).Tuning of acyl-ACP thioesterase activity directed for tailored fatty acid synthesis.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,102(7),3173-3182. |
| MLA | Feng, Yanbin,et al."Tuning of acyl-ACP thioesterase activity directed for tailored fatty acid synthesis".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.7(2018):3173-3182. |
入库方式： OAI收割
来源：大连化学物理研究所
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