Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks
文献类型：期刊论文
| 作者 | Dolley-Sonneville, Paula3; Peng, Jinlin1; Zhao, Yaopeng4; Hong, Yulong1; Burkhalter, Robert S.2; Hogue, Carrie L.2; Tran, Elizabeth1; Wei, Lai4; Romeo, Lori3; Melkoumian, Zara3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-04-03 |
| 卷号 | 90期号:7页码:4603-4610 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b05102 |
| 文献子类 | Article |
| 英文摘要 | This paper reports the chemical identity and mechanism of action and formation of a cell growth inhibitory compound leached from some single-use Erlenmeyer polycarbonate shaker flasks under routine cell culture conditions. Single-use cell culture vessels have been increasingly used for the production of biopharmaceuticals; however, they often suffer from issues associated with leachables that may interfere with cell growth and protein stability. Here, high-performance liquid-chromatography preparations and cell proliferation assays led to identification of a compound from the water extracts of some polycarbonate flasks, which exhibited subline- and seeding density-dependent growth inhibition of CHO cells in suspension culture. Mass spectroscopy, nuclear magnetic resonance spectroscopy, and chemical synthesis confirmed that this compound is 3,S-dinitro-bisphenol A. Cell cycle analysis suggests that 3,5-dinitro-bisphenol A arrests CHO-S cells at the G(1)/G(0) phase. Dynamic mass redistribution assays showed that 3,5-dinitro-bisphenol A is a weak GPR3S agonist. Analysis of the flask manufacturing process suggests that 3,5-dinitro-bisphenol A is formed via the combination of molding process with gamma-sterilization. This is the first report of a cell culture/assay interfering leachable compound that is formed through gamma-irradiation-mediated nitric oxide free radical reaction. |
| WOS关键词 | BISPHENOL-A POLYCARBONATE ; CONTAMINANTS LEACH ; DRUG DISCOVERY ; GPR35 AGONISTS ; PLASTIC TUBES ; CHO-CELLS ; GENOME ; IMPACT ; 3,3'-DINITROBISPHENOL ; IDENTIFICATION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000429385800051 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/168984]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Melkoumian, Zara; Liang, Xinmiao; Fang, Ye |
| 作者单位 | 1.Corning Inc, Corning R&D Corp, Biochem Technol, Corning, NY 14831 USA 2.Corning Inc, Corning R&D Corp, Characterizat Sci, Corning, NY 14831 USA 3.Corning Inc, Corning R&D Corp, Corning Life Sci Dev, Corning, NY 14831 USA 4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dolley-Sonneville, Paula,Peng, Jinlin,Zhao, Yaopeng,et al. Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks[J]. ANALYTICAL CHEMISTRY,2018,90(7):4603-4610. |
| APA | Dolley-Sonneville, Paula.,Peng, Jinlin.,Zhao, Yaopeng.,Hong, Yulong.,Burkhalter, Robert S..,...&Fang, Ye.(2018).Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks.ANALYTICAL CHEMISTRY,90(7),4603-4610. |
| MLA | Dolley-Sonneville, Paula,et al."Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks".ANALYTICAL CHEMISTRY 90.7(2018):4603-4610. |
入库方式： OAI收割
来源：大连化学物理研究所
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