Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells
文献类型：期刊论文
| 作者 | Wu, Huijian2; Sun, Guangwei3; Sun, Dongsheng1,2,3; Liu, Yang3; Wu, Hao1,3; Ren, Ying1,4; Ma, Xiaojun4 |
| 刊名 | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
 |
| 出版日期 | 2018-03-01 |
| 卷号 | 65期号:2页码:263-273 |
| 关键词 | Alginate Gel Bead Gelling Bath Mass Transfer Proliferation Three-dimensional Culture |
| ISSN号 | 0885-4513 |
| DOI | 10.1002/bab.1585 |
| 文献子类 | Article |
| 英文摘要 | Optimizing alginate gel beads is necessary to support the survival, proliferation, and function of entrapped hepatocytes. In this study, gelling bath was modified by decreasing calcium ion concentration and increasing sodium ion concentration. Alginate gel beads (using 36% G sodium alginate) prepared in the modified gelling bath had more homogeneous structure and better mass transfer properties compared with the traditional gelling bath that contains only calcium ions. Moreover, alginate gel beads generated in the modified gelling bath could significantly promote the HepG2 cell proliferation and the growth of cell spheroids, and maintain the albumin secretion ability similar to alginate gel beads prepared in the traditional gelling bath with only calcium ions. The mass transfer properties and cell proliferation were similar in ALG beads with different M/G ratio (36% G and 55% G) generated in the modified gelling bath, whereas they were significantly increased compared with alginate gel beads (55% G) in traditional gelling bath. These results indicated that adjusting the gelling bath was a simple and convenient method to enhance the mass transfer properties of alginate gel beads for 3D hepatocyte culture, which might provide more hepatocytes for the bioartificial liver support system. |
| WOS关键词 | BIOARTIFICIAL LIVER ; IMMOBILIZATION MATERIAL ; SPHEROID CULTURE ; C3A CELLS ; MICROCAPSULES ; MATRIX ; MODEL ; HEPATOCYTES ; SUPPORT ; POLYCATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000430115900019 |
| 出版者 | WILEY |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169036]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Wu, Huijian; Sun, Guangwei; Liu, Yang |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, 2 Ling Gong Rd, Dalian 116024, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Sci Res Ctr Translat Med, 457 Zhongshan Rd, Dalian 116023, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Huijian,Sun, Guangwei,Sun, Dongsheng,et al. Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2018,65(2):263-273. |
| APA | Wu, Huijian.,Sun, Guangwei.,Sun, Dongsheng.,Liu, Yang.,Wu, Hao.,...&Ma, Xiaojun.(2018).Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,65(2),263-273. |
| MLA | Wu, Huijian,et al."Effects of gelling bath on the physical properties of alginate gel beads and the biological characteristics of entrapped HepG2 cells".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 65.2(2018):263-273. |
入库方式： OAI收割
来源：大连化学物理研究所
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