Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells
文献类型：期刊论文
| 作者 | Wu, Huijian1; Zhao, Shan3; Ma, Xiaojun2; Sun, Guangwei3; Deng, Fei2,4; Zhang, Ying3; Sun, Dongsheng1,3,4; Liu, Yang3; Wang, Huihui3,4 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 109页码:1154-1163 |
| 关键词 | Hepatocellular Carcinoma Three-dimensional (3d) Cell Culture Decellularized Liver Matrix Alginate Gel Bead Urokinase Plasminogen Activator Matrix Metalloproteinase |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2017.11.103 |
| 文献子类 | Article |
| 英文摘要 | Developing reliable three-dimensional (3D) cell culture systems that can mimic native tumor microenvironments is necessary for investigating the mechanism of hepatocellular carcinoma (HCC) metastasis and screen therapeutic drugs. In the present study, we developed decellularized liver matrix-alginate (DLM-ALG) hybrid gel beads. DLM powder was prepared by optimized decellularization methods and liquid nitrogen grinding. DLM-ALG beads were generated by dropping alginate solution containing DLM powder into a gelling bath. DLM powder concentration in alginate solution was <= 1% (w/v) and had no effect on the sphericity and mechanical stability of the beads. In addition, HCCLM3 cells cultured in 1% (w/v) DLM-ALG beads presented gradually enhanced viability during in vitro culture. The protein expression of urokinase plasminogen activator system and activity of matrix metalloproteinases (MMPs) of HCCLM3 cells, including MMP2 and MMP9, were more significantly promoted in DLM-ALG beads compared with that in conventional ALG beads without DLM powder. Moreover, the dose-dependent increase in HCCLM3 cell MMP activities was observed along with the DLM powder concentration in 0.5% and 1% DLM-ALG groups. Therefore, DLM-ALG beads might serve as a novel 3D culture system for exploring the mechanisms of HCC metastasis and screening therapeutic drugs. (c) 2017 Elsevier B.V. All rights reserved. |
| WOS关键词 | PLASMINOGEN ACTIVATION SYSTEM ; EXTRACELLULAR-MATRIX ; COLORECTAL-CANCER ; IN-VITRO ; TUMOR MICROENVIRONMENT ; 3-DIMENSIONAL CULTURE ; SCAFFOLD MATERIAL ; TISSUE ; METALLOPROTEINASES ; EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000428099900125 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169046]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Sun, Guangwei; Liu, Yang |
| 作者单位 | 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Sci Res Ctr Translat Med, Dalian 116023, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Huijian,Zhao, Shan,Ma, Xiaojun,et al. Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,109:1154-1163. |
| APA | Wu, Huijian.,Zhao, Shan.,Ma, Xiaojun.,Sun, Guangwei.,Deng, Fei.,...&Wang, Huihui.(2018).Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,109,1154-1163. |
| MLA | Wu, Huijian,et al."Novel decellularized liver matrix-alginate hybrid gel beads for the 3D culture of hepatocellular carcinoma cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 109(2018):1154-1163. |
入库方式： OAI收割
来源：大连化学物理研究所
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