Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation
文献类型：期刊论文
| 作者 | Liang, Yu2; Wu, Ci1,2,3; Liang, Zhen2; Zhang, Lihua2; Zhang, Yukui2 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2018-08-17 |
| 卷号 | 1019页码:128-134 |
| 关键词 | Hybrid Monolith Mesoporosity Ethane-bridged Organosilane Peptide Separation Proteomics |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2018.02.033 |
| 文献子类 | Article |
| 英文摘要 | A novel kind of hybrid monolith with well-defined mesopore structure was prepared based on sol-gel condensation of 1, 2-bis(trimethoxysilyl) ethane (BTME) and tetramethoxysilane. Compared with terminal organosiloxanes used for preparation of conventional hybrid monoliths, BTME, as an ethanebridged alkoxysilane precursor, could not only maintain the uniformity of pore size distribution, but also improve the chemical stability of the monolith via Si-C bonds in the framework. Owing to the controllable mesoporous structure and good stability, the monolithic column was used for the separation of peptides with half peak width less than 6 s and the run-to-run and column-to-column relative standard deviations (RSD) for the retention time of five standard peptides less than 2.5%, showing narrow peak width and good reproducibility. Moreover, the separation performance could be well maintained even after washed by the mobile phase with pH 11.0 at 50 degrees C. Furthermore, 100 cm-length monolithic column was prepared and successfully used for nanoRPLC-ESI-MS/MS analysis of HeLa cell lysate digests, and 5670 proteins corresponding to 37574 peptides were identified from 750 ng of the sample, showing great promising of "single-shot" large-scale in-depth proteomic research. (c) 2018 Elsevier B.V. All rights reserved. |
| WOS关键词 | CAPILLARY LIQUID-CHROMATOGRAPHY ; ONE-POT PROCESS ; POROUS POLYMER MONOLITHS ; PEAK-CAPACITY ; SILICA MONOLITHS ; SMALL MOLECULES ; COLUMN ; LC ; ELECTROCHROMATOGRAPHY ; OPTIMIZATION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000429177600013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169088]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Inspect & Quarantine Tech Ctr Dalian Entry Exit I, Dalian 116100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yu,Wu, Ci,Liang, Zhen,et al. Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation[J]. ANALYTICA CHIMICA ACTA,2018,1019:128-134. |
| APA | Liang, Yu,Wu, Ci,Liang, Zhen,Zhang, Lihua,&Zhang, Yukui.(2018).Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation.ANALYTICA CHIMICA ACTA,1019,128-134. |
| MLA | Liang, Yu,et al."Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation".ANALYTICA CHIMICA ACTA 1019(2018):128-134. |
入库方式： OAI收割
来源：大连化学物理研究所
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