Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs
文献类型：期刊论文
| 作者 | Wang, Yaqing1,2; Su, Wentao1; Qin, Jianhua1,2; Jiang, Lei1; Liu, Yang1,2; Wang, Li1; Hui, Lijian2,3 |
| 刊名 | TOXICOLOGY RESEARCH
 |
| 出版日期 | 2018 |
| 卷号 | 7期号:1页码:13-21 |
| ISSN号 | 2045-452X |
| DOI | 10.1039/c7tx00209b |
| 文献子类 | Article |
| 英文摘要 | Preservation of hepatic phenotype and functions in vitro has always been a great challenge for the reconstruction of liver tissue engineering and in pharmaceutical research studies. Human induced hepatocytes (hiHeps) generated from fibroblasts can be reproducible with almost normal levels of liver specific functions, which are considered as a new source of hepatocytes for biomedical applications. Moreover, paper has served as an attractive biocompatible material for cell-based applications. In this study, we established a simple paper-based scaffold array for creating a 3D liver co-culture model that enabled the assessment of drug induced hepatotoxicity. The hiHeps co-cultured with HUVECs exhibited a 3D like morphology and maintained the liver specific functions of producing albumin and urea for up to 2 months. In addition, the hiHeps in this co-cultured model maintained a higher expression of cytochrome P450 genes as compared with a monolayer culture on a plate and a single culture on paper of hiHeps, revealing a marked enhancement of hepatic functions in the 3D liver co-culture model. Moreover, the 3D liver co-culture model was exposed to acetaminophen (APAP) and pioglitazone, exhibiting near physiological hepatotoxic responses compared to those of the monolayer cultures. Taken together, the low-cost and bioactive paper scaffold could offer great opportunities as 3D in vitro platforms for tissue engineering applications and high-throughput drug testing. |
| WOS关键词 | PRIMARY HUMAN HEPATOCYTES ; IN-VITRO SYSTEMS ; ON-A-CHIP ; ENDOTHELIAL-CELLS ; ENZYME-INDUCTION ; CULTURE ; METABOLISM ; TISSUE ; TRANSPORTER ; 2D |
| WOS研究方向 | Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000419189000002 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169176]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Qin, Jianhua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yaqing,Su, Wentao,Qin, Jianhua,et al. Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs[J]. TOXICOLOGY RESEARCH,2018,7(1):13-21. |
| APA | Wang, Yaqing.,Su, Wentao.,Qin, Jianhua.,Jiang, Lei.,Liu, Yang.,...&Hui, Lijian.(2018).Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs.TOXICOLOGY RESEARCH,7(1),13-21. |
| MLA | Wang, Yaqing,et al."Paper supported long-term 3D liver co-culture model for the assessment of hepatotoxic drugs".TOXICOLOGY RESEARCH 7.1(2018):13-21. |
入库方式： OAI收割
来源：大连化学物理研究所
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