A Thermophilic Tetramolecular G-Quadruplex/Hemin DNAzyme
文献类型：期刊论文
| 作者 | Ju, Huangxian1; Guo, Yuehua1; Chen, Jielin1; Cheng, Mingpan2; Monchaud, David3; Zhou, Jun1 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2017-12-22 |
| 卷号 | 56期号:52页码:16636-16640 |
| 关键词 | Dnazyme G-quadruplexes Hemin Peroxidase Activity Thermophilicity |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201708964 |
| 文献子类 | Article |
| 英文摘要 | The quadruplex-based DNAzyme system is one of the most useful artificial enzymes or catalysts; their unique properties make them reliable alternatives to proteins for performing catalytic transformation. The first prototype of a thermally stable DNAzyme system is presented. This thermophilic DNAzyme is capable of oxidizing substrates at high temperatures (up to 95 degrees C) and long reaction times (up to 18 h at 75 degrees C). The catalytic activity of the DNAzymes were investigated with the standard peroxidase-mimicking oxidation of 2,2'-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS) by H2O2. The step-by-step design of this unique heat-activated G-quadruplex/hemin catalyst, including the modification of adenines at both ends of G-tracts, the choice of cation, and its concentration for DNAzyme stabilization, is described. This work investigates thoroughly the molecular basis of these catalytic properties and provides an example of an industrially relevant application. |
| WOS关键词 | PEROXIDASE-MIMICKING DNAZYMES ; ACTIVITY ENHANCEMENT ; DNA ; SPECIFICITY ; OXIDATION ; INSIGHTS ; COMPLEX ; PORPHYRIN ; KINETICS ; APTAMERS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000418359400040 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169202]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ju, Huangxian; Zhou, Jun |
| 作者单位 | 1.Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 160023, Peoples R China 3.Univ Bourgogne ICMUB, CNRS, UMR6302, Inst Chim Mol, F-21000 Dijon, France |
| 推荐引用方式 GB/T 7714 | Ju, Huangxian,Guo, Yuehua,Chen, Jielin,et al. A Thermophilic Tetramolecular G-Quadruplex/Hemin DNAzyme[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(52):16636-16640. |
| APA | Ju, Huangxian,Guo, Yuehua,Chen, Jielin,Cheng, Mingpan,Monchaud, David,&Zhou, Jun.(2017).A Thermophilic Tetramolecular G-Quadruplex/Hemin DNAzyme.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(52),16636-16640. |
| MLA | Ju, Huangxian,et al."A Thermophilic Tetramolecular G-Quadruplex/Hemin DNAzyme".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.52(2017):16636-16640. |
入库方式： OAI收割
来源：大连化学物理研究所
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