Dual-Functionalized Magnetic Metal-Organic Framework for Highly Specific Enrichment of Phosphopeptides
文献类型：期刊论文
| 作者 | Zhou, Jianqiao1; Liang, Yulu1; He, Xiwen1; Chen, Langxing1,2; Zhang, Yukui1,3 |
| 刊名 | ACS SUSTAINABLE CHEMISTRY & ENGINEERING
 |
| 出版日期 | 2017-12-01 |
| 卷号 | 5期号:12页码:11413-11421 |
| ISSN号 | 2168-0485 |
| 关键词 | Phosphopeptide Enrichment Magnetic Nanomaterials Metal-organic Framework Immobilized Metal Ion Affinity Chromatography Metal Oxide Affinity Chromatography |
| DOI | 10.1021/acssuschemeng.7b02521 |
| 文献子类 | Article |
| 英文摘要 | The highly specific enrichment of phosphoproteins and phosphopeptides from intricate biological systems is the precondition of in-depth phosphoproteome research. Herein, a novel dual-functionalized magnetic zirconium-based metal-organic framework (MOF) denoted as DFMMOF, with the purpose of combining the affinity of immobilized metal ion affinity chromatography (IMAC) and metal oxide affinity chromatography (MOAC) has been successfully synthesized. The inherent Zr-O cluster of DFMMOF particles acted as MOAC and the immobilized titanium(IV) ions served for IMAC. The obtained DFMMOF exhibited rapid magnetic separation (within 5 s), large surface area (237.9 m(2) g(-1)), high binding capacity (100 mg g(-1)), and good postenrichment recovery (84.8%). Thanks to the strong affinity, low detection sensitivity (5 fmol) and high selectivity (beta-casein/BSA with a molar ratio of 1:1000) for phosphopeptide enrichment were obtained using DFMMOF as absorbent. Moreover, the effective identification of phosphopeptides from real samples (human serum and nonfat milk) further confirmed the immense potential of DFMMOF as a promising candidate for the detection and extraction of trace amounts of phosphorylated peptides in complex biosamples. |
| WOS关键词 | MALDI-TOF MS ; ION AFFINITY-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; SELECTIVE ENRICHMENT ; EFFICIENT ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; PROTEIN-PHOSPHORYLATION ; BASIC PHOSPHOPEPTIDES ; FACILE PREPARATION ; NANOPARTICLES |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Engineering |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000417341900032 |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169222]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Chen, Langxing |
| 作者单位 | 1.Nankai Univ, Tianjin Key Lab Biosensing & Mol Recognit, State Key Lab Med Chem Biol, Coll Chem, Tianjin 300071, Peoples R China 2.Nankai Univ, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Jianqiao,Liang, Yulu,He, Xiwen,et al. Dual-Functionalized Magnetic Metal-Organic Framework for Highly Specific Enrichment of Phosphopeptides[J]. ACS SUSTAINABLE CHEMISTRY & ENGINEERING,2017,5(12):11413-11421. |
| APA | Zhou, Jianqiao,Liang, Yulu,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).Dual-Functionalized Magnetic Metal-Organic Framework for Highly Specific Enrichment of Phosphopeptides.ACS SUSTAINABLE CHEMISTRY & ENGINEERING,5(12),11413-11421. |
| MLA | Zhou, Jianqiao,et al."Dual-Functionalized Magnetic Metal-Organic Framework for Highly Specific Enrichment of Phosphopeptides".ACS SUSTAINABLE CHEMISTRY & ENGINEERING 5.12(2017):11413-11421. |
入库方式： OAI收割
来源：大连化学物理研究所
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