Characterization, Quantification and Pharmacokinetic Analysis of Bevacizumab and Its Glycosylation by Mass Spectrometry
文献类型：期刊论文
| 作者 | Ye Ming-Liang2; Zou Han-Fa2; Cong Yu-Ting1,2; Hu Liang-Hai1; Gu Jing-Kai1 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 45期号:11页码:1678-1685 |
| 关键词 | Bevacizumab Glycosylation Liquid Chromatography-tandem Mass Spectrometry Parallel Reaction Monitoring Pharmacokinetics |
| ISSN号 | 0253-3820 |
| DOI | 10.11895/j.issn.0253-3820.170170 |
| 文献子类 | Article |
| 英文摘要 | The bevacizumab and its glycoforms were analyzed by matrix-assisted laser desorption ionization time of flight-time of flight-mass spectrometry (MALDI-TOF-MS), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and short-gun strategy, with the sequence of unique peptide and seventeen glycoforms being characterized. The bevacizumab and its glycopeptides concentrations in mice plasma with different intravenous injection doses of bevacizumab were detected and the concentration-time curves were obtained by parallel reaction monitoring (PRM) method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique. First, standard curves were created for quantification of mAb in mice plasma, which showed good linearity, with the correlation coefficient (R-2) value of 0.998 and the lower limit of quantification of 66 fmol. Detection results of high and low doses of the drug in the mice plasma samples showed that the drug concentration-time curve trend was consistent, e.g. the concentration was decreasing. However, the results of quantitation of seventeen glycoforms demonstrated that the metabolism of different glycoforms was different. The concentrations of most glycoforms increased first, whereas the metabolism afterwards differed by different glycoforms. |
| WOS关键词 | THERAPEUTIC ANTIBODIES ; IMMUNOGLOBULIN-G ; QUANTITATION ; PROTEOMICS ; MS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000419177900011 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169244]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Hu Liang-Hai |
| 作者单位 | 1.Jilin Univ, Coll Life Sci, Changchun 130012, Jilin, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye Ming-Liang,Zou Han-Fa,Cong Yu-Ting,et al. Characterization, Quantification and Pharmacokinetic Analysis of Bevacizumab and Its Glycosylation by Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2017,45(11):1678-1685. |
| APA | Ye Ming-Liang,Zou Han-Fa,Cong Yu-Ting,Hu Liang-Hai,&Gu Jing-Kai.(2017).Characterization, Quantification and Pharmacokinetic Analysis of Bevacizumab and Its Glycosylation by Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,45(11),1678-1685. |
| MLA | Ye Ming-Liang,et al."Characterization, Quantification and Pharmacokinetic Analysis of Bevacizumab and Its Glycosylation by Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 45.11(2017):1678-1685. |
入库方式： OAI收割
来源：大连化学物理研究所
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