DNA-Templated Aptamer Probe for Identification of Target Proteins
文献类型：期刊论文
| 作者 | Zhai, Guijin2; Tian, Shanshan2; Bi, Wenjing2; Bai, Xue2; Gao, Fan1; Zhang, Kai2; Zhang, Yukui1,5; Lu, Congcong1; Wang, Ye1; Fan, Enguo3,4 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-04-04 |
| 卷号 | 89期号:7页码:4071-4076 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b04895 |
| 文献子类 | Article |
| 英文摘要 | Using aptamers as molecular probes for biomarker discovery has attracted a great deal of attention in recent years. However, it is still a big challenge to accurately identify those protein markers that are targeted by aptamers under physiological conditions due to weak and noncovalent aptamer-protein interactions. Herein, we developed an aptamer based dual-probe using, DNA-templated chemistry and photo-cross-linking technique for the identification of target proteins that are recognized by aptamers. In this system, the aptamer was modified by a single strand DNA as binding probe (BP), and another complementary DNA with a photoactive group and reporter group was modified as capture probe (CP). BP was first added to recruit the binding protein via aptamer recognition, and Subsequently CP was added to let the cross-linker close to the target via DNA self-assembly, and then a covalent bond between CP and its binding protein was achieved via photo-cross-linking reaction. The captured protein can be detected or affinity enrichment using the tag, finally identified by MS. By use of lysozyme as a model substrate, we demonstrated that this multiple functionalized probe can be utilized for a successful labeling and enrichment of target protein even under a complicated and real environment. Thus, a novel method to precisely identify the aptamer-targeted proteins has been developed and it has a potential application for discovery of aptamer-based biomarkers. |
| WOS关键词 | BINDING PROTEINS ; CANCER-CELLS ; RNA APTAMER ; SELECTION ; APTASENSOR ; ENRICHMENT ; LYSOZYME ; LIGANDS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000398645300038 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169294]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Kai |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Tianjin Med Univ, Dept Biochem & Mol Biol, Tianjin Key Lab Med Epigenet, Key Lab Immune Microenvironm & Dis,Minist Educ, Tianjin 300070, Peoples R China 3.Univ Freiburg, Inst Biochem & Mol Biol, ZBMZ, Fac Med, D-79104 Freiburg, Germany 4.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Microbiol & Parasitol, Sch Basic Med,Peking Union Med Coll, Beijing 100005, Peoples R China 5.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhai, Guijin,Tian, Shanshan,Bi, Wenjing,et al. DNA-Templated Aptamer Probe for Identification of Target Proteins[J]. ANALYTICAL CHEMISTRY,2017,89(7):4071-4076. |
| APA | Zhai, Guijin.,Tian, Shanshan.,Bi, Wenjing.,Bai, Xue.,Gao, Fan.,...&Fan, Enguo.(2017).DNA-Templated Aptamer Probe for Identification of Target Proteins.ANALYTICAL CHEMISTRY,89(7),4071-4076. |
| MLA | Zhai, Guijin,et al."DNA-Templated Aptamer Probe for Identification of Target Proteins".ANALYTICAL CHEMISTRY 89.7(2017):4071-4076. |
入库方式： OAI收割
来源：大连化学物理研究所
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