Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c
文献类型：期刊论文
| 作者 | He, Xi-Wen1; Zhang, Yu-Kui1,3; Yan, Yun-Jing1; Li, Wen-You1,2 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2017-05-15 |
| 卷号 | 91页码:253-261 |
| 关键词 | Surface Imprinting Nitrogen-doped Graphene Quantum Dots Epitope Imprinting Metal Chelation Fluorescent Sensor |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2016.12.040 |
| 文献子类 | Article |
| 英文摘要 | A novel fluorescent sensor nitrogen-doped graphene quantum dots (N-GQDs)/SiO2/molecular imprinting polymer ( N-GQDs/SiO2/MIP) was fabricated by surface imprinting and epitope imprinting to recognize and detect the target protein cytochrome c (Cyt C) with fluorescence quenching. In the polymerization process, the C- and N-terminal nonapeptides of Cyt C were selected as the double templates which were fixed by functional monomer (zinc acrylate) through metal chelation and steady six-membered ring. The linear range of fluorescence quenching for this receptor towards Cyt C was 0.20-60 mu M, and the detection limit was 0.11 mu M. The precision for six times replicate determination of Cyt C at 30 M was 1.20%, and the imprinting factor (IF) was 3.06. The recoveries of the material to Cyt C in urine were 99.3-114.0%. In brief, this work proposed a strategy to prepare a new type fluorescent imprinting polymer based on N-GQDs and provided an attractive perspective for the detection of protein by using the combination of N-GQDs and molecular imprinting technique. |
| WOS关键词 | ONE-POT SYNTHESIS ; AQUEOUS-MEDIA ; FLUORESCENCE DETECTION ; PHENYLPHOSPHONIC ACID ; PROTEIN RECOGNITION ; SERUM-ALBUMIN ; BOVINE SERUM ; NANOPARTICLES ; SURFACE ; PEPTIDES |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000395599000038 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169326]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Yu-Kui; Li, Wen-You |
| 作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Coll Chem,Res Ctr Analyt Sci, Tianjin 300071, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Xi-Wen,Zhang, Yu-Kui,Yan, Yun-Jing,et al. Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c[J]. BIOSENSORS & BIOELECTRONICS,2017,91:253-261. |
| APA | He, Xi-Wen,Zhang, Yu-Kui,Yan, Yun-Jing,&Li, Wen-You.(2017).Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c.BIOSENSORS & BIOELECTRONICS,91,253-261. |
| MLA | He, Xi-Wen,et al."Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c".BIOSENSORS & BIOELECTRONICS 91(2017):253-261. |
入库方式： OAI收割
来源：大连化学物理研究所
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