Biphasic Affinity Chromatographic Approach for Deep Tyrosine Phosphoproteome Analysis
文献类型：期刊论文
| 作者 | Li, Shawn S. -C.1,4,5; Ye, Mingliang2; Deng, Zhenzhen2,3; Dong, Mingming2; Wang, Yan2,3; Dong, Jing2; Zou, Hanfa2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-02-21 |
| 卷号 | 89期号:4页码:2405-2410 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b04288 |
| 文献子类 | Article |
| 英文摘要 | Tyrosine phosphorylation (pTyr) is important for normal physiology and implicated in many human diseases, particularly cancer. Identification of pTyr sites is critical to dissecting signaling pathways and understanding disease pathologies. However, compared with serine/threonine phosphorylation (pSer/pThr), the analysis of pTyr at the proteome level is more challenging due to its low abundance. Here, we developed a biphasic affinity chromatographic approach where Src SH2 superbinder was coupled with NeutrAvidin affinity chromatography, for tyrosine phosphoproteome analysis. With the use of competitive elution agent biotin-pYEEI, this strategy can distinguish high-affinity phosphotyrosyl peptides from low-affinity ones, while the excess competitive agent is readily removed by using NeutrAvidin agarose resin in an integrated tip system. The excellent performance of this system was demonstrated by analyzing tyrosine phosphoproteome of Jurkat cells from which 3,480 unique pTyr sites were identified. The biphasic affinity chromatography method for deep Tyr phosphoproteome analysis is rapid, sensitive, robust, and cost-effective. It is widely applicable to the global analysis of the tyrosine phosphoproteome associated with tyrosine kinase signal transduction. |
| WOS关键词 | SH2 DOMAINS ; IDENTIFICATION ; SUPERBINDER ; PROTEOME ; REVEALS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000394724700034 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169336]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ye, Mingliang |
| 作者单位 | 1.Western Univ, Dept Oncol, London, ON N6A 5C1, Canada 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 1000491, Peoples R China 4.Western Univ, Dept Biochem, London, ON N6A 5C1, Canada 5.Western Univ, Childrens Hlth Res Inst, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada |
| 推荐引用方式 GB/T 7714 | Li, Shawn S. -C.,Ye, Mingliang,Deng, Zhenzhen,et al. Biphasic Affinity Chromatographic Approach for Deep Tyrosine Phosphoproteome Analysis[J]. ANALYTICAL CHEMISTRY,2017,89(4):2405-2410. |
| APA | Li, Shawn S. -C..,Ye, Mingliang.,Deng, Zhenzhen.,Dong, Mingming.,Wang, Yan.,...&Zou, Hanfa.(2017).Biphasic Affinity Chromatographic Approach for Deep Tyrosine Phosphoproteome Analysis.ANALYTICAL CHEMISTRY,89(4),2405-2410. |
| MLA | Li, Shawn S. -C.,et al."Biphasic Affinity Chromatographic Approach for Deep Tyrosine Phosphoproteome Analysis".ANALYTICAL CHEMISTRY 89.4(2017):2405-2410. |
入库方式： OAI收割
来源：大连化学物理研究所
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