NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing
文献类型：期刊论文
| 作者 | Liang, Zhen1; Zhang, Shen1,2,3; Shan, Yichu1; Zhang, Shurong1; Sui, Zhigang1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2017-02-10 |
| 卷号 | 154页码:40-48 |
| 关键词 | De Novo Sequencing Non-isobaric Peptide Termini Labeling Quantitative Proteomics Mass-defect Based Labeling Peptide Mutation |
| ISSN号 | 1874-3919 |
| DOI | 10.1016/j.jprot.2016.12.003 |
| 文献子类 | Article |
| 英文摘要 | A simple and effective de novo sequencing strategy assisted by non-isobaric peptide termini labeling, NIPTL-Novo, was established. The y-series ions and b-series ions of peptides can be clearly distinguished according to the different mass tags incorporated in N-terminus and C-terminus. This is helpful for improving the accuracy of peptide sequencing and increasing the sequencing speed. For the spectra commonly identified by both de novo sequencing and database searching software (Mascot or Maxquant), NIPTL-Novo gave identical result to more than 85% of these spectra. Furthermore, the quantitative profiling of the sample can be performed simultaneously along with de novo sequencing. Finally, this strategy can be applied to discover the peptides with potential mutation sites by combining with mass-defect based isotopic labeling. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; PROTEOME QUANTIFICATION ; QUANTITATIVE PROTEOMICS ; IDENTIFICATION ; ACQUISITION ; ALGORITHM ; DIGESTION ; ION ; HCC ; MS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000394191200005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169380]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zhang, Lihua |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, Toronto, ON, Canada |
| 推荐引用方式 GB/T 7714 | Liang, Zhen,Zhang, Shen,Shan, Yichu,et al. NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing[J]. JOURNAL OF PROTEOMICS,2017,154:40-48. |
| APA | Liang, Zhen.,Zhang, Shen.,Shan, Yichu.,Zhang, Shurong.,Sui, Zhigang.,...&Zhang, Yukui.(2017).NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing.JOURNAL OF PROTEOMICS,154,40-48. |
| MLA | Liang, Zhen,et al."NIPTL-Novo: Non-isobaric peptide termini labeling assisted peptide de novo sequencing".JOURNAL OF PROTEOMICS 154(2017):40-48. |
入库方式： OAI收割
来源：大连化学物理研究所
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