Phosphoproteome Profiling reveals circadian clock regulation of Posttranslational Modifications in the Murine hippocampus
文献类型：期刊论文
| 作者 | Cheng, Hai-Ying Mary3; Chiang, Cheng-Kang1,2; Xu, Bo1; Mehta, Neel3; Mayne, Janice1; Sun, Warren Y. L.1; Cheng, Kai1; Ning, Zhibin1; Dong, Jing4; Zou, Hanfa4 |
| 刊名 | FRONTIERS IN NEUROLOGY
 |
| 出版日期 | 2017-03-22 |
| 卷号 | 8 |
| 关键词 | Hippocampus Circadian Rhythm Quantitative Proteome And phosphoProteome Analysis Phosphorylation Kinase-substrate Relations |
| ISSN号 | 1664-2295 |
| DOI | 10.3389/fneur.2017.00110 |
| 文献子类 | Article |
| 英文摘要 | The circadian clock is an endogenous oscillator that drives daily rhythms in physiology, behavior, and gene expression. The underlying mechanisms of circadian timekeeping are cell-autonomous and involve oscillatory expression of core clock genes that is driven by interconnecting transcription-translation feedback loops (TTFLs). Circadian clock TTFLs are further regulated by posttranslational modifications, in particular, phosphorylation. The hippocampus plays an important role in spatial memory and the conversion of short-to long-term memory. Several studies have reported the presence of a peripheral oscillator in the hippocampus and have highlighted the importance of circadian regulation in memory formation. Given the general importance of phosphorylation in circadian clock regulation, we performed global quantitative proteome and phosphoproteome analyses of the murine hippocampus across the circadian cycle, applying spiked-in labeled reference and high accuracy mass spectrometry (MS). Of the 3,052 proteins and 2,868 phosphosites on 1,368 proteins that were accurately quantified, 1.7% of proteins and 5.2% of phosphorylation events exhibited time-of-day-dependent expression profiles. The majority of circadian phosphopeptides displayed abrupt fluctuations at mid-to-late day without underlying rhythms of protein abundance. Bioinformatic analysis of cyclic phosphorylation events revealed their diverse distribution in different biological pathways, most notably, cytoskeletal organization and neuronal morphogenesis. This study provides the first large-scale, quantitative MS analysis of the circadian phosphoproteome and proteome of the murine hippocampus and highlights the significance of rhythmic regulation at the posttranslational level in this peripheral oscillator. In addition to providing molecular insights into the hippocampal circadian clock, our results will assist in the understanding of genetic factors that underlie rhythms-associated pathological states of the hippocampus. |
| WOS关键词 | CAMP-RESPONSIVE ELEMENT ; MEMORY-PERSISTENCE ; MOUSE-LIVER ; PROTEIN ; PHOSPHORYLATION ; KINASE ; NETWORKS ; RHYTHMS ; CORTICOSTERONE ; OSCILLATION |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000396978200001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169422]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Cheng, Hai-Ying Mary; Figeys, Daniel |
| 作者单位 | 1.Univ Ottawa, Ottawa Inst Syst Biol, Dept Biochem Microbiol & Immunol, Fac Med, Ottawa, ON, Canada 2.Natl Dong Hwa Univ, Dept Chem, Hualien, Taiwan 3.Univ Toronto, Dept Biol, Mississauga, ON, Canada 4.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 5.Univ Ottawa, Dept Chem & Biomol Sci, Ottawa, ON, Canada 6.Canadian Inst Adv Res, Toronto, ON, Canada |
| 推荐引用方式 GB/T 7714 | Cheng, Hai-Ying Mary,Chiang, Cheng-Kang,Xu, Bo,et al. Phosphoproteome Profiling reveals circadian clock regulation of Posttranslational Modifications in the Murine hippocampus[J]. FRONTIERS IN NEUROLOGY,2017,8. |
| APA | Cheng, Hai-Ying Mary.,Chiang, Cheng-Kang.,Xu, Bo.,Mehta, Neel.,Mayne, Janice.,...&Figeys, Daniel.(2017).Phosphoproteome Profiling reveals circadian clock regulation of Posttranslational Modifications in the Murine hippocampus.FRONTIERS IN NEUROLOGY,8. |
| MLA | Cheng, Hai-Ying Mary,et al."Phosphoproteome Profiling reveals circadian clock regulation of Posttranslational Modifications in the Murine hippocampus".FRONTIERS IN NEUROLOGY 8(2017). |
入库方式： OAI收割
来源：大连化学物理研究所
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