Chromatographic methods for the analysis of oligosaccharides in human milk
文献类型：期刊论文
| 作者 | Yan, Jingyu1; Liang, Xinmiao1; Ding, Junjie1,2 |
| 刊名 | ANALYTICAL METHODS
 |
| 出版日期 | 2017-02-21 |
| 卷号 | 9期号:7页码:1071-1077 |
| ISSN号 | 1759-9660 |
| DOI | 10.1039/c6ay02982e |
| 文献子类 | Review |
| 英文摘要 | Human milk oligosaccharides (HMOs) are important components in human milk because of their high concentrations and benefits to infant growth. To better understand the relationship between oligosaccharide structures and biofunctions, efficient qualitative and quantitative methods are essential prerequisites. However, HMOs are highly complex and difficult to analyze because of their high polarity, lack of chromophores, and the presence of multiple isomeric structures. Although advanced mass spectrometry can provide much detailed structural information, chromatography is still important to reduce the complexity of HMOs in mixtures. The objective of this review is to describe and discuss the recent advances in the analysis of HMOs with the applications of different chromatographic methods, including gel permeation, high performance anion-exchange, porous graphitized carbon, hydrophilic interaction, reversed-phase high performance liquid chromatography, and capillary electrophoresis. These methods have been used to characterize the structures of HMOs, profile oligosaccharides obtained from the milk of different mothers, and measure the concentration changes in the course of lactations. For each method, we focus on the separation principles, commonly used separation conditions, typical applications, and the ability to resolve the isomers. |
| WOS关键词 | HYDROPHILIC INTERACTION CHROMATOGRAPHY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; IONIZATION-MASS-SPECTROMETRY ; THIN-LAYER-CHROMATOGRAPHY ; SAMPLE PREPARATION METHOD ; CAPILLARY-ELECTROPHORESIS ; NEUTRAL OLIGOSACCHARIDES ; SIALYLATED OLIGOSACCHARIDES ; STRUCTURAL DETERMINATION ; H-1-NMR SPECTROSCOPY |
| WOS研究方向 | Chemistry ; Food Science & Technology ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000395684800001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169432]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Liang, Xinmiao |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Jingyu,Liang, Xinmiao,Ding, Junjie. Chromatographic methods for the analysis of oligosaccharides in human milk[J]. ANALYTICAL METHODS,2017,9(7):1071-1077. |
| APA | Yan, Jingyu,Liang, Xinmiao,&Ding, Junjie.(2017).Chromatographic methods for the analysis of oligosaccharides in human milk.ANALYTICAL METHODS,9(7),1071-1077. |
| MLA | Yan, Jingyu,et al."Chromatographic methods for the analysis of oligosaccharides in human milk".ANALYTICAL METHODS 9.7(2017):1071-1077. |
入库方式： OAI收割
来源：大连化学物理研究所
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