Determination of N-Glycopeptides by Hydrophilic Interaction Liquid Chromatography and Porous Graphitized Carbon Chromatography with Mass Spectrometry Detection
文献类型：期刊论文
| 作者 | Zou, Lijuan1; Lu, Jun1; Fu, Dongmei2; Yu, Long2; Cao, Cuiyan2; Liang, Xinmiao2 |
| 刊名 | ANALYTICAL LETTERS
 |
| 出版日期 | 2017 |
| 卷号 | 50期号:2页码:315-324 |
| 关键词 | Glycan Glycopeptide Hydrophilic Interaction Chromatography Mass Spectrometry Porous Graphitized Carbon |
| ISSN号 | 0003-2719 |
| DOI | 10.1080/00032719.2016.1181644 |
| 文献子类 | Article |
| 英文摘要 | An offline two-dimensional chromatographic method based on the combination of hydrophilic interaction liquid chromatography (HILIC) and porous graphitized carbon (PGC) chromatography was developed for the separation and purification of glycopeptides. The high selectivity of HILIC and PGC isolated high-purity isomers of N-glycopeptides from ribonuclease B. N-Glycopeptides were first separated from nonglycosylated peptides, and N-glycopeptides were sorted into fractions through the first-dimensional HILIC according to their monosaccharides. Further separation of the glycopeptide isomers in each fraction was achieved using second-dimensional PGC. Structural differences of the glycopeptide isomers were further enzymatically hydrolyzed with peptide-N-glycosidase F. The glycan structure were elucidated by matrix assisted laser desorption ionization tandem quadrupole time-of-flight mass spectrometry. The established procedure allows the isolation of glycopeptide or glycan standards from natural sources. |
| WOS关键词 | LINKED GLYCANS ; GLYCOSYLATION ; PHASE ; GLYCOPROTEINS ; ELECTROSPRAY ; ENRICHMENT |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000395072100005 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169542]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Zou, Lijuan; Yu, Long |
| 作者单位 | 1.Dalian Med Univ, Dept Radiol, Affiliated Hosp 2, Dalian, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Lijuan,Lu, Jun,Fu, Dongmei,et al. Determination of N-Glycopeptides by Hydrophilic Interaction Liquid Chromatography and Porous Graphitized Carbon Chromatography with Mass Spectrometry Detection[J]. ANALYTICAL LETTERS,2017,50(2):315-324. |
| APA | Zou, Lijuan,Lu, Jun,Fu, Dongmei,Yu, Long,Cao, Cuiyan,&Liang, Xinmiao.(2017).Determination of N-Glycopeptides by Hydrophilic Interaction Liquid Chromatography and Porous Graphitized Carbon Chromatography with Mass Spectrometry Detection.ANALYTICAL LETTERS,50(2),315-324. |
| MLA | Zou, Lijuan,et al."Determination of N-Glycopeptides by Hydrophilic Interaction Liquid Chromatography and Porous Graphitized Carbon Chromatography with Mass Spectrometry Detection".ANALYTICAL LETTERS 50.2(2017):315-324. |
入库方式： OAI收割
来源：大连化学物理研究所
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