中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study

文献类型:期刊论文

作者Gu, Jingkai2; Cong, Yuting1,2; Hu, Lianghai2; Zhang, Zhang1; Gao, Yin2; Dong, Mingming1; Qin, Hongqiang1; Ye, Mingliang1; Zou, Hanfa1
刊名TALANTA
出版日期2017-04-01
卷号165页码:664-670
关键词Proteomics Mass Spectrometry Therapeutic Monoclonal Antibody Glycosylation Parallel Reaction Monitoring Pharmacokinetics
ISSN号0039-9140
DOI10.1016/j.talanta.2017.01.023
文献子类Article
英文摘要Monoclonal antibodies (mAbs), are one of the most important protein drugs have attracted increasing attention. However, the pharmacokinetics of mAbs has not been fully investigated due to the complexity of protein drugs. Traditonal immuno-based approaches can not recognize the proteoforms of mAbs because of the long development cycles, prohibitive cost, and interactions between different proteins. Therefore, reliable qualitative and quantitative analysis of the proteoforms of mAbs in biological samples is of crucial importance. Herein, a novel method was developed for absolute quantitation of mAbs and their glycoforms in complex biological samples such as serum and tissues. With the combination of HILIC enrichment and parallel reaction monitoring by high resolution mass spectrometry, most of the glycoforms can be accurately quantified at the fmol level through the use of the model mAb of bevacizumab. More importantly, the structural confirmation can be achieved simultaneously without the need for additional experiments. This strategy can be readily applied to the pharmacokinetic study of glycosylation modification and biomarker discovery for clinical applications.
WOS关键词IMMUNOGLOBULIN-G ; TARGETED PROTEOMICS ; HIGH-RESOLUTION ; HUMAN SERUM ; FC ; GLYCOPEPTIDES ; ACCURATE ; GLYCANS
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000394064700094
出版者ELSEVIER SCIENCE BV
源URL[http://cas-ir.dicp.ac.cn/handle/321008/169566]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者Hu, Lianghai; Ye, Mingliang
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Jilin Univ, Sch Life Sci, Changchun 130012, Peoples R China
推荐引用方式
GB/T 7714
Gu, Jingkai,Cong, Yuting,Hu, Lianghai,et al. Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study[J]. TALANTA,2017,165:664-670.
APA Gu, Jingkai.,Cong, Yuting.,Hu, Lianghai.,Zhang, Zhang.,Gao, Yin.,...&Zou, Hanfa.(2017).Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study.TALANTA,165,664-670.
MLA Gu, Jingkai,et al."Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study".TALANTA 165(2017):664-670.

入库方式: OAI收割

来源:大连化学物理研究所

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