Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study
文献类型:期刊论文
作者 | Gu, Jingkai2; Cong, Yuting1,2; Hu, Lianghai2; Zhang, Zhang1; Gao, Yin2; Dong, Mingming1; Qin, Hongqiang1; Ye, Mingliang1; Zou, Hanfa1 |
刊名 | TALANTA
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出版日期 | 2017-04-01 |
卷号 | 165页码:664-670 |
关键词 | Proteomics Mass Spectrometry Therapeutic Monoclonal Antibody Glycosylation Parallel Reaction Monitoring Pharmacokinetics |
ISSN号 | 0039-9140 |
DOI | 10.1016/j.talanta.2017.01.023 |
文献子类 | Article |
英文摘要 | Monoclonal antibodies (mAbs), are one of the most important protein drugs have attracted increasing attention. However, the pharmacokinetics of mAbs has not been fully investigated due to the complexity of protein drugs. Traditonal immuno-based approaches can not recognize the proteoforms of mAbs because of the long development cycles, prohibitive cost, and interactions between different proteins. Therefore, reliable qualitative and quantitative analysis of the proteoforms of mAbs in biological samples is of crucial importance. Herein, a novel method was developed for absolute quantitation of mAbs and their glycoforms in complex biological samples such as serum and tissues. With the combination of HILIC enrichment and parallel reaction monitoring by high resolution mass spectrometry, most of the glycoforms can be accurately quantified at the fmol level through the use of the model mAb of bevacizumab. More importantly, the structural confirmation can be achieved simultaneously without the need for additional experiments. This strategy can be readily applied to the pharmacokinetic study of glycosylation modification and biomarker discovery for clinical applications. |
WOS关键词 | IMMUNOGLOBULIN-G ; TARGETED PROTEOMICS ; HIGH-RESOLUTION ; HUMAN SERUM ; FC ; GLYCOPEPTIDES ; ACCURATE ; GLYCANS |
WOS研究方向 | Chemistry |
语种 | 英语 |
WOS记录号 | WOS:000394064700094 |
出版者 | ELSEVIER SCIENCE BV |
源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169566] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
通讯作者 | Hu, Lianghai; Ye, Mingliang |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Jilin Univ, Sch Life Sci, Changchun 130012, Peoples R China |
推荐引用方式 GB/T 7714 | Gu, Jingkai,Cong, Yuting,Hu, Lianghai,et al. Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study[J]. TALANTA,2017,165:664-670. |
APA | Gu, Jingkai.,Cong, Yuting.,Hu, Lianghai.,Zhang, Zhang.,Gao, Yin.,...&Zou, Hanfa.(2017).Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study.TALANTA,165,664-670. |
MLA | Gu, Jingkai,et al."Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study".TALANTA 165(2017):664-670. |
入库方式: OAI收割
来源:大连化学物理研究所
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