Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy
文献类型：期刊论文
| 作者 | Chen, Rui; Qin, Hongqiang; Cheng, Kai; Zhu, Jun; Mao, Jiawei; Wang, Fangjun; Dong, Mingming; Guo, Zhimou; Liang, Xinmiao; Ye, Mingliang |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-02-07 |
| 卷号 | 89期号:3页码:1469-1476 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b02887 |
| 文献子类 | Article |
| 英文摘要 | The diversity of O-linked glycan structures has drawn increasing attention due to its vital biological roles. However, intact O-glycopeptides with different glycans are typically not well elucidated using the current methods. In this work, an integrated strategy was developed for comprehensive analysis of O-GalNAc glycosylation by combining hydrophilic interaction chromatography (HILIC) tip enrichment, beam-type collision induced decomposition (beam-CID) detection, and in silico deglycosylation method for spectra interpretation. In this strategy, the intact O-GalNAc glycopeptides were selectively enriched and the original spectra obtained by time-of-flight (TOF)-CID were preprocessed using an in silico deglycosylation method, enabling direct searching without setting multiple glycosylation modifications, which could significantly decrease the search space. This strategy was applied to analyze the O-GalNAc glycoproteome of human serum, leading to identification of 407 intact O-GalNAc glycopeptides from 93 glycoproteins. About 81% of the glycopeptides contained at least one sialic acid, which could reveal the microheterogeneity of O-GalNAc glycosylation. Up until now, this is the largest data set of intact O-GalNAc glycoforms from complex biological samples at the proteome level. Furthermore, this method is readily applicable to study O-glycoform heterogeneity in other complex biological systems. |
| WOS关键词 | SITE-SPECIFIC CHARACTERIZATION ; HUMAN CEREBROSPINAL-FLUID ; SOLID-PHASE EXTRACTION ; LINKED GLYCOPEPTIDES ; MASS-SPECTROMETRY ; N-GLYCOSYLATION ; PLASMA-PROTEINS ; OVARIAN-CANCER ; LC-MS/MS ; IDENTIFICATION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000393738300014 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://cas-ir.dicp.ac.cn/handle/321008/169606]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 通讯作者 | Ye, Mingliang |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Rui,Qin, Hongqiang,Cheng, Kai,et al. Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy[J]. ANALYTICAL CHEMISTRY,2017,89(3):1469-1476. |
| APA | Chen, Rui.,Qin, Hongqiang.,Cheng, Kai.,Zhu, Jun.,Mao, Jiawei.,...&Zou, Hanfa.(2017).Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy.ANALYTICAL CHEMISTRY,89(3),1469-1476. |
| MLA | Chen, Rui,et al."Proteomics Analysis of O-GaINAc Glycosylation in Human Serum by an Integrated Strategy".ANALYTICAL CHEMISTRY 89.3(2017):1469-1476. |
入库方式： OAI收割
来源：大连化学物理研究所
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